PMID- 2155834
OWN - NLM
STAT- MEDLINE
DCOM- 19900419
LR  - 20190813
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 68
IP  - 2-3
DP  - 1990 Jan 22
TI  - Regulation of ACTH-induced steroidogenesis in human fetal adrenals by rTNF-alpha.
PG  - R31-6
AB  - The presence of tumor necrosis factor type alpha (TNF-alpha) in different fetal 
      tissue and adult adrenal extracts was investigated by radioimmunoassay (RIA). 
      Measurable levels of TNF-alpha were found in 12/22 fetal adrenals, but in none of 
      the seven adult adrenals studied. Since it is known that (i) steroidogenesis in 
      fetal adrenals differs greatly from that in adult glands by having higher 
      androgen/corticosteroid ratio, (ii) and that macrophage-derived factors may cause 
      adrenocortical suppression, the effect of TNF-alpha on corticotropin-induced 
      steroidogenesis in primary cultures of human fetal adrenals was studied. Results 
      show that TNF-alpha effectively suppresses the production of cortisol and shifts 
      the steroid synthesis towards androgen production. The effect was not accompanied 
      by any change in cell viability and could be neutralized by addition of 
      polyclonal rabbit anti-TNF-alpha antiserum to cell cultures. These results 
      suggest that TNF-alpha may take part in the regulation of human fetal 
      steroidogenesis within the network of the fetoplacental unit via inhibition of 
      the cortisol synthesis.
FAU - Jaattela, M
AU  - Jaattela M
AD  - Department of Pathology, University of Helsinki, Finland.
FAU - Carpen, O
AU  - Carpen O
FAU - Stenman, U H
AU  - Stenman UH
FAU - Saksela, E
AU  - Saksela E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Androgens)
RN  - 0 (Steroids)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9002-60-2 (Adrenocorticotropic Hormone)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Adrenal Glands/drug effects/*metabolism
MH  - Adrenocorticotropic Hormone/*pharmacology
MH  - Androgens/biosynthesis
MH  - Culture Techniques
MH  - Humans
MH  - Hydrocortisone/biosynthesis
MH  - Steroids/*biosynthesis
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 1990/01/22 00:00
MHDA- 1990/01/22 00:01
CRDT- 1990/01/22 00:00
PHST- 1990/01/22 00:00 [pubmed]
PHST- 1990/01/22 00:01 [medline]
PHST- 1990/01/22 00:00 [entrez]
AID - 0303-7207(90)90196-F [pii]
AID - 10.1016/0303-7207(90)90196-f [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 1990 Jan 22;68(2-3):R31-6. doi: 
      10.1016/0303-7207(90)90196-f.