PMID- 21559831
OWN - NLM
STAT- MEDLINE
DCOM- 20120501
LR  - 20220330
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Print)
IS  - 0301-4851 (Linking)
VI  - 39
IP  - 2
DP  - 2012 Feb
TI  - Association between TNF-alpha promoter G-308A and G-238A polymorphisms and obesity.
PG  - 825-9
LID - 10.1007/s11033-011-0804-4 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha), an adipokine, is produced in adipocytes, and the 
      elevation of its levels has been linked to obesity and insulin resistance in some 
      population. In this study the relationship between TNF-alpha promoter gene 
      polymorphism and obesity in an Iranian population has been studied. Subjects were 
      randomly selected from Tehran Cohort Lipid and Glucose Study. Adult participants 
      placed in three groups according to their body mass index (BMI): BMI < 25, 25 </= 
      BMI < 30, BMI >/= 30 and under-18 subjects placed in two groups, under 85th 
      percentile BMI and above 85th percentile. Finally, 244 persons were selected for 
      G-308A and G-238A polymorphisms analysis. The FBS, high-density lipoprotein 
      cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), triglyceride, 
      cholesterol levels and blood pressure and HOMA of all subjects were measured. The 
      polymorphism -308 and -238 were revealed by restriction fragment length 
      polymorphism (RFLP; NCOI and MSPI) after the promoter site was amplified by PCR. 
      The allele frequency of TNF-alpha polymorphism was in the Hardy-Weinberg equilibrium. 
      There was no relation between BMI and the frequency of this allele. The fact that 
      there is no association between G-308A and G-238A TNF-alpha promoter polymorphisms 
      and obesity probably shows that it is not an important risk factor for obesity 
      and consequently for cardiovascular disease.
FAU - Hedayati, M
AU  - Hedayati M
AD  - Obesity Research Center, Research Institute for Endocrine Sciences, Shahid 
      Beheshti University of Medical Sciences, 19395-4763 Tehran, Iran. 
      Hedayati@endocrine.ac.ir
FAU - Sharifi, K
AU  - Sharifi K
FAU - Rostami, F
AU  - Rostami F
FAU - Daneshpour, M S
AU  - Daneshpour MS
FAU - Zarif Yeganeh, M
AU  - Zarif Yeganeh M
FAU - Azizi, F
AU  - Azizi F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110511
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Triglycerides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Blood Pressure
MH  - Body Mass Index
MH  - Cholesterol/blood
MH  - Gene Frequency
MH  - Genetic Association Studies
MH  - Humans
MH  - Iran/epidemiology
MH  - Obesity/*epidemiology/*genetics
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - Polymorphism, Single Nucleotide/*genetics
MH  - Promoter Regions, Genetic/*genetics
MH  - Triglycerides/genetics
MH  - Tumor Necrosis Factor-alpha/*genetics
PMC - PMC3249554
EDAT- 2011/05/12 06:00
MHDA- 2012/05/02 06:00
PMCR- 2011/05/11
CRDT- 2011/05/12 06:00
PHST- 2010/08/14 00:00 [received]
PHST- 2011/04/29 00:00 [accepted]
PHST- 2011/05/12 06:00 [entrez]
PHST- 2011/05/12 06:00 [pubmed]
PHST- 2012/05/02 06:00 [medline]
PHST- 2011/05/11 00:00 [pmc-release]
AID - 804 [pii]
AID - 10.1007/s11033-011-0804-4 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2012 Feb;39(2):825-9. doi: 10.1007/s11033-011-0804-4. Epub 2011 May 
      11.