PMID- 21561324
OWN - NLM
STAT- MEDLINE
DCOM- 20120522
LR  - 20120201
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 21
IP  - 3
DP  - 2012 Feb 10
TI  - Recruitment of stem cells into the injured retina after laser injury.
PG  - 448-54
LID - 10.1089/scd.2011.0002 [doi]
AB  - Retinal degeneration is a devastating complication of diabetes and other 
      disorders. Stem cell therapy for retinal degeneration has shown encouraging 
      results but functional regeneration has not been yet achieved. Our study was 
      undertaken to evaluate the localization of stem cells delivered to the retina by 
      intravenous versus intravitreal infusion, because stem cell localization is a key 
      factor in ultimate in vivo function. We used lineage-negative bone marrow-derived 
      stem cells in a model wherein retina of mice was induced by precise and 
      reproducible laser injury. Lin(-ve) bone marrow cells (BMCs) were labeled with a 
      tracking dye and their homing capacity was analyzed at time points after 
      infusion. We found that Lin(-ve) BMCs get incorporated into laser-injured retina 
      when transplanted through either the intravitreal or intravenous route. The 
      intravenous route resulted in optimal localization of donor cells at the site of 
      injury. These cells incorporated into injured retina in a dose-dependent manner. 
      The data presented in this study reflect the importance of dose and route for 
      stem cell-based treatment designed to result in retinal regeneration.
FAU - Singh, Tajinder
AU  - Singh T
AD  - Department of Neurology, Post Graduate Institute of Medical Education and 
      Research, Chandigarh, India.
FAU - Prabhakar, Sudesh
AU  - Prabhakar S
FAU - Gupta, Amod
AU  - Gupta A
FAU - Anand, Akshay
AU  - Anand A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110728
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Fluorescent Dyes)
RN  - 9009-81-8 (Rhodopsin)
SB  - IM
MH  - Angiography/methods
MH  - Animals
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - Bone Marrow Transplantation/*methods
MH  - Cell Differentiation
MH  - Disease Models, Animal
MH  - Female
MH  - Fluorescent Dyes
MH  - Injections, Intravenous
MH  - Intravitreal Injections
MH  - Lasers/adverse effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Regeneration
MH  - Retina/*injuries/metabolism/*transplantation
MH  - Retinal Degeneration/pathology/therapy
MH  - Rhodopsin/metabolism
MH  - Staining and Labeling
EDAT- 2011/05/13 06:00
MHDA- 2012/05/23 06:00
CRDT- 2011/05/13 06:00
PHST- 2011/05/13 06:00 [entrez]
PHST- 2011/05/13 06:00 [pubmed]
PHST- 2012/05/23 06:00 [medline]
AID - 10.1089/scd.2011.0002 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2012 Feb 10;21(3):448-54. doi: 10.1089/scd.2011.0002. Epub 2011 
      Jul 28.