PMID- 21561609
OWN - NLM
STAT- MEDLINE
DCOM- 20110829
LR  - 20110704
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 96
IP  - 1
DP  - 2011 Jul
TI  - Sperm and embryo analysis of similar t(7;10) translocations transmitted in two 
      families.
PG  - e66-70
LID - 10.1016/j.fertnstert.2011.04.042 [doi]
AB  - OBJECTIVE: To compare the sperm meiotic segregation profiles in two men from 
      families with similar t(7;10) translocations and determine the frequency of 
      unbalanced sperm and preimplantation embryos in one couple. DESIGN: Analysis of 
      sperm nuclei and blastomeres by fluorescence in situ hybridization (FISH). 
      SETTING: Research institute. PATIENT(S): Carriers of balanced translocations 
      t(7;10)(q34;q24) and t(7;10)(q36;q24.3). INTERVENTION(S): Multicolor FISH using 
      probes for chromosomes 7, 10, 8, 18, 21, X, and Y on sperm and preimplantation 
      genetic diagnosis (PGD) of blastomeres. MAIN OUTCOME MEASURE(S): Frequencies of 
      meiotic segregation products in sperm and blastomeres and sperm aneuploidy of 
      chromosomes 8, 18, 21, X, and Y. RESULT(S): Similar meiotic segregation patterns, 
      with preferential alternate segregation (50.6% in patient P1, 48.1% in P2) 
      followed by adjacent 1, adjacent 2 and 3:1 segregations, were observed in the 
      sperm of the two carriers. An interchromosomal effect on the sex chromosomes was 
      found when compared with disomy frequencies reported in control donors. The 
      results of preimplantation genetic diagnosis in the first couple are roughly 
      consistent with the sperm analysis results. CONCLUSION(S): Carriers of similar 
      translocations show similar segregation profiles. The in vitro fertilization 
      method accompanied by preimplantation genetic diagnosis increases the chance of 
      translocation carriers fathering a healthy child.
CI  - Copyright (c) 2011 American Society for Reproductive Medicine. Published by 
      Elsevier Inc. All rights reserved.
FAU - Vozdova, Miluse
AU  - Vozdova M
AD  - Department of Genetics and Reproduction, Veterinary Research Institute, Brno, 
      Czech Republic. vozdova@vri.cz
FAU - Oracova, Eva
AU  - Oracova E
FAU - Musilova, Petra
AU  - Musilova P
FAU - Kasikova, Katerina
AU  - Kasikova K
FAU - Prinosilova, Petra
AU  - Prinosilova P
FAU - Gaillyova, Renata
AU  - Gaillyova R
FAU - Rubes, Jiri
AU  - Rubes J
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110511
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
MH  - Adult
MH  - Chromosomes, Human, Pair 10/*genetics
MH  - Chromosomes, Human, Pair 7/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Pedigree
MH  - Preimplantation Diagnosis/*methods
MH  - Spermatozoa/*physiology
MH  - Translocation, Genetic/*genetics
EDAT- 2011/05/13 06:00
MHDA- 2011/08/30 06:00
CRDT- 2011/05/13 06:00
PHST- 2011/01/27 00:00 [received]
PHST- 2011/04/11 00:00 [revised]
PHST- 2011/04/13 00:00 [accepted]
PHST- 2011/05/13 06:00 [entrez]
PHST- 2011/05/13 06:00 [pubmed]
PHST- 2011/08/30 06:00 [medline]
AID - S0015-0282(11)00655-8 [pii]
AID - 10.1016/j.fertnstert.2011.04.042 [doi]
PST - ppublish
SO  - Fertil Steril. 2011 Jul;96(1):e66-70. doi: 10.1016/j.fertnstert.2011.04.042. Epub 
      2011 May 11.