PMID- 21562444
OWN - NLM
STAT- MEDLINE
DCOM- 20111129
LR  - 20211020
IS  - 1536-4828 (Electronic)
IS  - 0885-3177 (Print)
IS  - 0885-3177 (Linking)
VI  - 40
IP  - 6
DP  - 2011 Aug
TI  - Mesenchymal stem cells facilitate mixed hematopoietic chimerism induction and 
      prevent onset of diabetes in nonobese diabetic mice.
PG  - 846-54
LID - 10.1097/MPA.0b013e318215cdce [doi]
AB  - OBJECTIVES: Allogeneic mesenchymal stem cells (MSCs) and bone marrow cells (BMCs) 
      were cotransplanted in nonobese diabetic mice after none myeloablative 
      preconditioning and the development of chimerism, insulitis, diabetes, and 
      graft-versus-host disease (GVHD) were monitored. METHODS: Eight-week-old female 
      nonobese diabetic mice were injected intravenously with 2 x 10 BMCs and 5 x 10 
      MSCs from C57BL/6 mice after treatment with 2 intraperitoneal injections of 
      anti-CD3 antibody (days -7 and -4) and 3-Gy total body irradiation (day -1). 
      Thereafter, blood glucose and chimerism were monitored on peripheral blood 
      samples. RESULTS: Stable mixed chimerism (3->90% of donor phenotype) was induced 
      in 63.2% of BMCs-MSCs recipients (n = 19) and 45.0% of BMCs-alone recipients (n = 
      20, P = 0.256). Insulitis was prevented, and euglycemia persisted for more than 
      18 weeks in 89.5% of BMCs-MSCs recipients including those with less than 3% 
      chimerism and 55% of BM-alone recipients (P < 0.05). In controls, 9.1% of mice 
      receiving preconditioning treatment alone (n = 11) and 16.7% of preconditioned 
      mice receiving only MSCs (n = 12) were nondiabetic. Graft-versus-host disease was 
      not detected in all mice. CONCLUSIONS: Coinjection of MSCs and BMCs increased the 
      success rate in inducing chimerism and preventing insulitis and overt diabetes 
      with no incidence of GVHD. Results also indicated that even microchimerism with 
      less than 3% donor cells is sufficient for blocking autoimmunity.
FAU - Asari, Sadaki
AU  - Asari S
AD  - Southern California Islet Cell Resources Center, Department of Diabetes, 
      Endocrinology and Metabolism, Beckman Research Institute of City of Hope, Duarte, 
      CA, USA.
FAU - Itakura, Shin
AU  - Itakura S
FAU - Rawson, Jeffrey
AU  - Rawson J
FAU - Ito, Taihei
AU  - Ito T
FAU - Todorov, Ivan
AU  - Todorov I
FAU - Nair, Indu
AU  - Nair I
FAU - Shintaku, Jonathan
AU  - Shintaku J
FAU - Liu, Chih-Pin
AU  - Liu CP
FAU - Kandeel, Fouad
AU  - Kandeel F
FAU - Mullen, Yoko S
AU  - Mullen YS
LA  - eng
GR  - U42 RR016607/RR/NCRR NIH HHS/United States
GR  - U42 RR016607-08/RR/NCRR NIH HHS/United States
GR  - U42RR16607/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Pancreas
JT  - Pancreas
JID - 8608542
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation/immunology
MH  - Diabetes Mellitus, Type 1/*prevention & control
MH  - Female
MH  - Graft vs Host Disease/prevention & control
MH  - Immune Tolerance
MH  - Islets of Langerhans/cytology/immunology
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred CBA
MH  - Mice, Inbred NOD
MH  - *Transplantation Chimera/immunology
MH  - Transplantation Conditioning
MH  - Transplantation, Homologous
PMC - PMC3138826
MID - NIHMS282173
EDAT- 2011/05/13 06:00
MHDA- 2011/12/13 00:00
PMCR- 2012/08/01
CRDT- 2011/05/13 06:00
PHST- 2011/05/13 06:00 [entrez]
PHST- 2011/05/13 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2012/08/01 00:00 [pmc-release]
AID - 10.1097/MPA.0b013e318215cdce [doi]
PST - ppublish
SO  - Pancreas. 2011 Aug;40(6):846-54. doi: 10.1097/MPA.0b013e318215cdce.