PMID- 21566367
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110901
LR  - 20220317
IS  - 1342-6311 (Print)
IS  - 1342-6311 (Linking)
VI  - 24
IP  - 2
DP  - 2009
TI  - Characterization of active microbes in a full-scale anaerobic fluidized bed 
      reactor treating phenolic wastewater.
PG  - 144-53
AB  - This study investigated the active microbial community in a full-scale granular 
      activated carbon-anaerobic fluidized bed (GAC-AFB) reactor treating wastewater 
      from the manufacturing of phenolic resin, using 16S rRNA-based molecular 
      analyses. The results of cDNA from 16S rRNA revealed that Methanosaeta-related 
      (83.9% of archaeal clones) and Syntrophorhabdaceae (formerly named 
      Deltaproteobacteria group TA)-related (68.9% of bacterial clones) microorganisms 
      were as the most predominant populations in the phenol-degrading GAC-AFB reactor. 
      The high abundance of Syntrophorhabdaceae was supported by a terminal restriction 
      fragment length polymorphism (T-RFLP) analysis, which showed that a 
      Syntrophorhabdaceae-like fragment of 119 bp (~80% of total fragments) was the 
      most predominant phylotype. Furthermore, fluorescence in situ hybridization 
      (FISH) analyses suggested that Syntrophus- and Chloroflexi-like cells were also 
      in high abundance in the GAC biofilm. A non-layered structure of microorganisms 
      was found in the GAC biofilm, where Methanosaeta (thick filamentous), 
      Syntrophorhabdaceae (oval-shaped), Syntrophus (small rods) and Chloroflexi 
      (thin-filamentous) were randomly distributed with high abundance. These findings 
      greatly improve our understanding of the diversity and distribution of microbial 
      populations in a full-scale mesophilic bioreactor treating an actual 
      phenol-containing waste stream.
FAU - Chen, Chia-Lung
AU  - Chen CL
AD  - Department of Civil Engineering, National University of Singapore, Block E1A 
      #07-03, Engineering Drive 2, Singapore 117576.
FAU - Wu, Jer-Horng
AU  - Wu JH
FAU - Tseng, I-Cheng
AU  - Tseng IC
FAU - Liang, Teh-Ming
AU  - Liang TM
FAU - Liu, Wen-Tso
AU  - Liu WT
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Microbes Environ
JT  - Microbes and environments
JID - 9710937
EDAT- 2009/01/01 00:00
MHDA- 2009/01/01 00:01
CRDT- 2011/05/14 06:00
PHST- 2011/05/14 06:00 [entrez]
PHST- 2009/01/01 00:00 [pubmed]
PHST- 2009/01/01 00:01 [medline]
AID - JST.JSTAGE/jsme2/ME09109 [pii]
AID - 10.1264/jsme2.me09109 [doi]
PST - ppublish
SO  - Microbes Environ. 2009;24(2):144-53. doi: 10.1264/jsme2.me09109.