PMID- 2156717
OWN - NLM
STAT- MEDLINE
DCOM- 19900507
LR  - 20190707
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 187
IP  - 2
DP  - 1990 Apr
TI  - Localization of urokinase-type plasminogen activator receptor on U937 cells: 
      phorbol ester PMA induces heterogeneity.
PG  - 255-62
AB  - The binding of human urokinase-type plasminogen activator (u-PA) to the surface 
      of the human monocytic cell line U937 was studied by immunological detection of 
      bound u-PA or binding of biotinylated diisopropyl fluorophosphate-inactivated 
      human u-PA visualized by light or electron microscopy. Untreated U937 cells 
      showed a characteristic binding pattern, with the majority of the u-PA bound to 
      the microvillar-containing protruding pole of the cells. After treatment with the 
      phorbol ester PMA, the resulting adherent cell population was very heterogeneous 
      with respect to both cellular morphology and u-PA binding. The bound u-PA was 
      distributed on both the dorsal and the substrate side of the cells, and the 
      patches of bound u-PA could not be correlated to any typical membrane 
      conformations or cell-cell or cell-substratum contacts. When a monoclonal 
      antibody directed against the amino-terminal fragment (ATF) of u-PA was used, the 
      results were identical regardless of whether intact u-PA or ATF was used for 
      binding to the cells. In contrast, when a monoclonal antibody recognizing the 
      non-receptor-binding protease domain of u-PA was used, bound ATF showed no 
      staining, while bound intact u-PA was stained as efficiently as above. The 
      alteration of u-PA receptor distribution following treatment with PMA could be 
      related to the changes in glycosylation and ligand affinity of the purified u-PA 
      receptor previously described following PMA treatment of U937 cells.
FAU - Hansen, S H
AU  - Hansen SH
AD  - Finsen Laboratory, Rigshospitalet, Copenhagen, Denmark.
FAU - Behrendt, N
AU  - Behrendt N
FAU - Dano, K
AU  - Dano K
FAU - Kristensen, P
AU  - Kristensen P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Enzyme Precursors)
RN  - 0 (PLAUR protein, human)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Urokinase Plasminogen Activator)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Cell Line
MH  - Cell Membrane/metabolism/ultrastructure
MH  - Dexamethasone/pharmacology
MH  - Enzyme Precursors/metabolism
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Immunohistochemistry
MH  - Lymphoma, Large B-Cell, Diffuse
MH  - Microscopy, Electron
MH  - Receptors, Cell Surface/*analysis/drug effects/ultrastructure
MH  - Receptors, Urokinase Plasminogen Activator
MH  - Tetradecanoylphorbol Acetate/*pharmacology
MH  - Tumor Cells, Cultured/cytology/drug effects/*metabolism
MH  - Urokinase-Type Plasminogen Activator/metabolism
EDAT- 1990/04/01 00:00
MHDA- 1990/04/01 00:01
CRDT- 1990/04/01 00:00
PHST- 1990/04/01 00:00 [pubmed]
PHST- 1990/04/01 00:01 [medline]
PHST- 1990/04/01 00:00 [entrez]
AID - 10.1016/0014-4827(90)90089-s [doi]
PST - ppublish
SO  - Exp Cell Res. 1990 Apr;187(2):255-62. doi: 10.1016/0014-4827(90)90089-s.