PMID- 215672
OWN - NLM
STAT- MEDLINE
DCOM- 19790324
LR  - 20191210
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 26
IP  - 12
DP  - 1978 Dec
TI  - Cytochemical demonstration of sodium, potassium-adenosine triphosphatase by a 
      hemepeptide derivative of ouabain.
PG  - 1042-52
AB  - A cytochemical probe for the ultrastructural localization of the NaKATPase was 
      devised, which utilizes the biological affinity of the noncompetitive inhibitor, 
      ouabain (ouab) to which was coupled a hemepeptide (H11P) which possesses 
      peroxidatic activity. The conjugate, ouab-H11P, had an apparent Ki of 
      approximately 8 x 10(-7) M. When reacted with fixed tissue from the salt gland of 
      osmotically stressed ducklings, the NaKATPase was localized to the basal and 
      lateral infoldings of the plasma membranes of secretory epithelial cells. 
      Reaction product consisted of fine textured deposits distributed in focal patches 
      on the outer aspects of the membrane. Apical membranes were negative, as were 
      intracellular membrane components. Preincubation of tissue with unlabeled ouabain 
      or binding of ouab-H11P in the presence of 10 mM K+, no ATP and no Mg++, resulted 
      in the absence or diminution of reaction product.
FAU - Mazurkiewicz, J E
AU  - Mazurkiewicz JE
FAU - Hossler, F E
AU  - Hossler FE
FAU - Barrnett, R J
AU  - Barrnett RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Hemeproteins)
RN  - 5ACL011P69 (Ouabain)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
SB  - IM
MH  - Animals
MH  - Cell Membrane/enzymology
MH  - Ducks
MH  - Epithelium/enzymology
MH  - Hemeproteins
MH  - Histocytochemistry
MH  - Ouabain/*analogs & derivatives
MH  - Salt Gland/*enzymology
MH  - Sodium-Potassium-Exchanging ATPase/*isolation & purification
EDAT- 1978/12/01 00:00
MHDA- 1978/12/01 00:01
CRDT- 1978/12/01 00:00
PHST- 1978/12/01 00:00 [pubmed]
PHST- 1978/12/01 00:01 [medline]
PHST- 1978/12/01 00:00 [entrez]
AID - 10.1177/26.12.215672 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1978 Dec;26(12):1042-52. doi: 10.1177/26.12.215672.