PMID- 21567938
OWN - NLM
STAT- MEDLINE
DCOM- 20111115
LR  - 20211020
IS  - 1552-4930 (Electronic)
IS  - 1552-4922 (Print)
IS  - 1552-4922 (Linking)
VI  - 79
IP  - 8
DP  - 2011 Aug
TI  - Semi-automated scoring of triple-probe FISH in human sperm: methods and further 
      validation.
PG  - 661-6
LID - 10.1002/cyto.a.21078 [doi]
AB  - Although the frequency and consequence of sperm chromosomal abnormalities are 
      considerable, few epidemiologic studies in large samples have been conducted to 
      investigate etiologic risk factors. This is, in part, attributable to the labor 
      intensive demands of manual sperm fluorescence in situ hybridization (FISH) 
      scoring. As part of an epidemiologic study investigating environmental risk 
      factors for aneuploidy among men attending a hospital-based fertility clinic, a 
      semi-automated method of slide scoring was further validated and used to estimate 
      sex chromosome sperm disomy frequency in a large number of samples. Multiprobe 
      FISH for chromosomes X, Y, and 18 was used to determine sex chromosome disomy in 
      sperm nuclei. Semi-automated scoring methods were used to quantify X disomy 
      (sperm FISH genotype XX18), Y disomy (YY18), and XY disomy (XY18). The 
      semi-automated results were compared with the results from manual scoring in 10 
      slides. The semi-automated method was then used to estimate sex chromosome disomy 
      frequency in 60 men. Of 10 slides scored, significant differences between the 
      manual and semi-automated results were seen primarily in one slide that was of 
      poor quality because of over swollen nuclei. Among 60 men analyzed using the 
      semi-automated method, median total sex chromosome disomy frequency was 1.65%, 
      which is higher than seen among normal men but within range with reports from 
      fertility clinic populations. These results further validate that semi-automated 
      methods can be used to score sperm disomy with results comparable to manual 
      methods. This is the largest study to date to provide estimates of sex chromosome 
      disomy among men attending fertility clinics. These methods should be replicated 
      in larger clinic populations to arrive at stable estimates of aneuploidy 
      frequency in men who are members of subfertile couples. (c) 2011 International 
      Society for Advancement of Cytometry.
CI  - Copyright (c) 2011 International Society for Advancement of Cytometry.
FAU - Perry, Melissa J
AU  - Perry MJ
AD  - Department of Environmental and Occupational Health, The George Washington 
      University School of Public Health and Health Services, Washington, DC 20037, 
      USA. melissa.perry@gwumc.edu
FAU - Chen, Xing
AU  - Chen X
FAU - McAuliffe, Megan E
AU  - McAuliffe ME
FAU - Maity, Arnab
AU  - Maity A
FAU - Deloid, Glen M
AU  - Deloid GM
LA  - eng
GR  - R00 ES017744/ES/NIEHS NIH HHS/United States
GR  - T42 OH008416/OH/NIOSH CDC HHS/United States
GR  - P30 ES000002/ES/NIEHS NIH HHS/United States
GR  - R01 ES009718/ES/NIEHS NIH HHS/United States
GR  - R01 ES009718-01A1/ES/NIEHS NIH HHS/United States
GR  - P30ES000002/ES/NIEHS NIH HHS/United States
GR  - KO1ES10959/ES/NIEHS NIH HHS/United States
GR  - K01 ES010959-01/ES/NIEHS NIH HHS/United States
GR  - K01 ES010959/ES/NIEHS NIH HHS/United States
GR  - P30 ES000002-50/ES/NIEHS NIH HHS/United States
GR  - R01ES09718/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110512
PL  - United States
TA  - Cytometry A
JT  - Cytometry. Part A : the journal of the International Society for Analytical 
      Cytology
JID - 101235694
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - *Aneuploidy
MH  - Chromosomes, Human, Pair 18/genetics
MH  - Chromosomes, Human, X/genetics
MH  - Chromosomes, Human, Y/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - *Sex Chromosome Aberrations
MH  - Spermatozoa/*pathology
MH  - Uniparental Disomy/*cytology
PMC - PMC3349235
MID - NIHMS305833
EDAT- 2011/05/14 06:00
MHDA- 2011/11/16 06:00
PMCR- 2012/08/01
CRDT- 2011/05/14 06:00
PHST- 2010/11/02 00:00 [received]
PHST- 2011/03/18 00:00 [revised]
PHST- 2011/04/12 00:00 [accepted]
PHST- 2011/05/14 06:00 [entrez]
PHST- 2011/05/14 06:00 [pubmed]
PHST- 2011/11/16 06:00 [medline]
PHST- 2012/08/01 00:00 [pmc-release]
AID - 10.1002/cyto.a.21078 [doi]
PST - ppublish
SO  - Cytometry A. 2011 Aug;79(8):661-6. doi: 10.1002/cyto.a.21078. Epub 2011 May 12.