PMID- 21569703
OWN - NLM
STAT- MEDLINE
DCOM- 20120402
LR  - 20110516
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 32
IP  - 4
DP  - 2011 Apr
TI  - [Application of FICTION technique to the detection of genetic aberrations in 
      multiple myeloma].
PG  - 226-30
AB  - OBJECTIVE: To investigate the diagnostic value of FICTION (Fluorescence 
      Immunophenotyping and Interphase Cytogenetics as a Tool for the Investigation of 
      Neoplasms) technique, combining immunofluorescence and fluorescence in situ 
      hybridization (FISH), to detect genetic aberrations in multiple myeloma (MM). 
      METHODS: Bone marrow samples were collected from 18 MM and 2 plasma cell leukemia 
      (PCL) patients. Probes targeting IgH and MMSET were prepared using a Nick 
      Translation Kit from Bacterial artificial chromosome (BAC) clones. The 
      immunophenotyping was achieved via the CD138 tyramide signal amplification 
      (TSA)-mediated immunofluorescence, followed by FISH with the prepared probes 
      \[t(4;14), t(11;14), t(14;16)\] and the commercial deletion probes (13q and p53) 
      to detect common genetic aberrations in MM. RESULTS: All the 20 samples were 
      assayed with the probes mentioned above, and revealed 4 cases with t(4;14), 6 
      with t(11;14), 1 with t(14;16), 3 with p53 deletion; and 8 with 13q deletion. The 
      remaining 4 cases had none of the 5 aberrations. CONCLUSION: FICTION technique 
      facilitates the detection of genetic abnormalities of MM in situ; enhances both 
      efficiency and sensitivity of positive detection, thus, could be used as the 
      screening test of molecular diagnosis of MM to guide coming-up risk-adapted 
      therapy and evaluate prognosis.
FAU - Wang, Di
AU  - Wang D
AD  - Department of Hematology, Huazhong University of Science and Technology, Wuhan 
      430030, China.
FAU - Huang, Liang
AU  - Huang L
FAU - Zhang, Heng
AU  - Zhang H
FAU - Geng, Zhe
AU  - Geng Z
FAU - Shang, Zhen
AU  - Shang Z
FAU - Zhou, Jian-feng
AU  - Zhou JF
FAU - Li, Chun-rui
AU  - Li CR
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cytogenetics
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*diagnosis/*genetics
EDAT- 2011/05/17 06:00
MHDA- 2012/04/03 06:00
CRDT- 2011/05/17 06:00
PHST- 2011/05/17 06:00 [entrez]
PHST- 2011/05/17 06:00 [pubmed]
PHST- 2012/04/03 06:00 [medline]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2011 Apr;32(4):226-30.