PMID- 21569830
OWN - NLM
STAT- MEDLINE
DCOM- 20111214
LR  - 20220409
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 136
IP  - 2
DP  - 2011 Jun 22
TI  - Protective effect of GCSB-5, an herbal preparation, against peripheral nerve 
      injury in rats.
PG  - 297-304
LID - 10.1016/j.jep.2011.04.037 [doi]
AB  - AIM OF THE STUDY: GCSB-5 (traditional name: Chungpa-Juhn), an herbal medicine 
      composed of 6 crude herbs (Saposhnikovia divaricata Schiskin, Achyranthis 
      bidentata Blume, Acanthopanax sessiliflorum Seem, Cibotium baromets J. Smith, 
      Glycine max Meriill, and Eucommia ulmoides Oliver), has been widely used in Asia 
      for treatment of neuropathic and inflammatory diseases. This study investigated 
      the protective effect of GCSB-5 against peripheral nerve injury in vitro and in 
      vivo. MATERIALS AND METHODS: After left sciatic nerve transection, rats received 
      oral administration of GCSB-5 (30, 100, 300, and 600 mg/kg), or saline (vehicle), 
      respectively, once daily for 8 weeks. Motor functional recovery and axonal nerve 
      regeneration were evaluated by measurement of sciatic functional index (SFI), 
      sensory regeneration distance, and gastrocnemius muscle mass ratio. The 
      myelinated axon number was counted by morphometric analysis. In the in vitro 
      study, the effects of GCSB-5 on H(2)O(2)-induced oxidative damage in SH-SY5Y 
      cells were investigated by measurement of cell viability, production of reactive 
      oxygen species (ROS), lipid peroxidation, release of lactate dehydrogenease 
      (LDH), and cellular glutathione contents. Neurite outgrowth was also determined. 
      RESULTS: After 8 weeks of nerve transection, SFI, regeneration distance, and 
      gastrocnemius muscle mass ratio and myelinated axon number showed a significant 
      decrease and these decreases were attenuated by GCSB-5. GCSB-5 significantly 
      inhibited H(2)O(2)-induced cell death and oxidative stress, as evidenced by 
      decreases in production of ROS and lipid peroxidation and release of LDH, and by 
      increase in total GSH content. CONCLUSIONS: The neuroprotective effect afforded 
      by GCSB-5 is due in part to reduced oxidative stress.
CI  - Copyright (c) 2011 Elsevier Ireland Ltd. All rights reserved.
FAU - Kim, Tae-Hoon
AU  - Kim TH
AD  - School of Pharmacy, Sungkyunkwan University, Suwon 440-746, South Korea.
FAU - Yoon, Seong-Jin
AU  - Yoon SJ
FAU - Lee, Woo-Cheol
AU  - Lee WC
FAU - Kim, Joon-Ki
AU  - Kim JK
FAU - Shin, Joonshik
AU  - Shin J
FAU - Lee, Sangho
AU  - Lee S
FAU - Lee, Sun-Mee
AU  - Lee SM
LA  - eng
PT  - Journal Article
DEP - 20110504
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Antioxidants)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism/pharmacology/*therapeutic use
MH  - Cell Death/drug effects
MH  - Drugs, Chinese Herbal/pharmacology/*therapeutic use
MH  - Glutathione/metabolism
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Male
MH  - Motor Activity/drug effects
MH  - Muscle, Skeletal/drug effects/pathology
MH  - Nerve Fibers/drug effects
MH  - Nerve Regeneration/*drug effects/physiology
MH  - Neuroprotective Agents/pharmacology/therapeutic use
MH  - Oxidative Stress/drug effects
MH  - Peripheral Nervous System Diseases/*drug therapy/pathology/physiopathology
MH  - *Phytotherapy
MH  - Plants, Medicinal
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/metabolism
MH  - Sciatic Nerve/*drug effects/pathology/physiopathology
MH  - Trauma, Nervous System/*drug therapy/pathology/physiopathology
EDAT- 2011/05/17 06:00
MHDA- 2011/12/15 06:00
CRDT- 2011/05/17 06:00
PHST- 2011/01/11 00:00 [received]
PHST- 2011/04/12 00:00 [revised]
PHST- 2011/04/14 00:00 [accepted]
PHST- 2011/05/17 06:00 [entrez]
PHST- 2011/05/17 06:00 [pubmed]
PHST- 2011/12/15 06:00 [medline]
AID - S0378-8741(11)00277-7 [pii]
AID - 10.1016/j.jep.2011.04.037 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2011 Jun 22;136(2):297-304. doi: 10.1016/j.jep.2011.04.037. 
      Epub 2011 May 4.