PMID- 21570059
OWN - NLM
STAT- MEDLINE
DCOM- 20120224
LR  - 20111003
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 56
IP  - 10
DP  - 2011 Oct
TI  - Expression of HMGB1 and HMGN2 in gingival tissues, GCF and PICF of periodontitis 
      patients and peri-implantitis.
PG  - 1106-11
LID - 10.1016/j.archoralbio.2011.03.020 [doi]
AB  - BACKGROUND AND OBJECTIVE: High mobility group chromosomal protein B1 (HMGB1) and 
      N2 (HMGN2), two members of high mobility group (HMG) family, play important role 
      in inflammation. The purpose of this study was to investigate the expression of 
      HMGB1 and HMGN2 in periodontistis. MATERIALS AND METHODS: The expression of HMGB1 
      and HMGN2 mRNA in gingival tissues and gingival crevicular fluid (GCF) in chronic 
      periodontitis (CP), generalised aggressive periodontitis (G-AgP) patients and 
      healthy subjects was detected by real-time PCR. The protein level of HMGB1 and 
      HMGN2 in peri-implant crevicular fluid (PICF), peri-implant crevicular fluid of 
      peri-implantitis (PI-PICF) and normal patients was determined by Western 
      blotting. Furthermore, IL-1beta, IL-6, IL-8, TNF-alpha and HMGB1 levels in GCF, PI-PICF 
      and healthy-PICF samples from different groups were determined by ELISA. RESULTS: 
      HMGN2 expression was increased in inflamed gingival tissues and GCF from CP and 
      G-ApG groups compared to control group. HMGB1 expression was the highest in the 
      gingival tissues and GCF from CP patients and was accompanied by increased 
      concentrations of IL-1beta, IL-6, IL-8 proinflammaory cytokines. CONCLUSION: To our 
      knowledge, this is the first study reporting that the expression of HMGB1 and 
      HMGN2 was increased in the gingival tissues and GCF in CP and G-AgP and the PICF 
      in PICF. Our data suggest that HMGB1 may be a potential target for the therapy of 
      periodontitis and PI.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Luo, Lin
AU  - Luo L
AD  - Department of anesthesiology, West China College of Stomatology, Sichuan 
      University, Chengdu, China.
FAU - Xie, Ping
AU  - Xie P
FAU - Gong, Ping
AU  - Gong P
FAU - Tang, Xiao-hai
AU  - Tang XH
FAU - Ding, Yi
AU  - Ding Y
FAU - Deng, Lu-Xia
AU  - Deng LX
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110512
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Dental Implants)
RN  - 0 (HMGB1 Protein)
RN  - 0 (HMGN2 Protein)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aggressive Periodontitis/metabolism
MH  - Alveolar Bone Loss/metabolism
MH  - Chronic Periodontitis/metabolism
MH  - Dental Calculus/metabolism
MH  - *Dental Implants
MH  - Dental Plaque Index
MH  - Female
MH  - Gingiva/*metabolism
MH  - Gingival Crevicular Fluid/*chemistry
MH  - Gingival Hemorrhage/metabolism
MH  - Gingivitis/metabolism
MH  - HMGB1 Protein/*analysis
MH  - HMGN2 Protein/*analysis
MH  - Humans
MH  - Inflammation Mediators/analysis
MH  - Interleukin-1beta/analysis
MH  - Interleukin-6/analysis
MH  - Interleukin-8/analysis
MH  - Male
MH  - Middle Aged
MH  - Peri-Implantitis/*metabolism
MH  - Periodontal Pocket/metabolism
MH  - Periodontitis/*metabolism
MH  - Periodontium/metabolism
MH  - Tumor Necrosis Factor-alpha/analysis
MH  - Young Adult
EDAT- 2011/05/17 06:00
MHDA- 2012/03/01 06:00
CRDT- 2011/05/17 06:00
PHST- 2010/12/02 00:00 [received]
PHST- 2011/02/24 00:00 [revised]
PHST- 2011/03/25 00:00 [accepted]
PHST- 2011/05/17 06:00 [entrez]
PHST- 2011/05/17 06:00 [pubmed]
PHST- 2012/03/01 06:00 [medline]
AID - S0003-9969(11)00100-2 [pii]
AID - 10.1016/j.archoralbio.2011.03.020 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2011 Oct;56(10):1106-11. doi: 10.1016/j.archoralbio.2011.03.020. 
      Epub 2011 May 12.