PMID- 21571960
OWN - NLM
STAT- MEDLINE
DCOM- 20110712
LR  - 20141120
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 135
IP  - 6
DP  - 2011 Jun
TI  - Inaccuracy of a "spiked curve" for monitoring unfractionated heparin therapy.
PG  - 870-6
LID - 10.1309/AJCP60ZGXCJKRMJO [doi]
AB  - Our purpose was to determine whether the in vitro (or "spiked curve") method for 
      the therapeutic heparin range is an accurate and valid method for heparin 
      monitoring. Many laboratories use the in vitro method for determining the 
      activated partial thromboplastin time (APTT)-based unfractionated heparin (UFH) 
      therapeutic range as a more practical method to determine the therapeutic heparin 
      range. Is this a valid method compared with the recommended ex vivo method? 
      Plasma samples from patients receiving UFH and a normal plasma pool spiked with 
      UFH were compared for 8 APTT reagents (18 lots). The in vitro curve has 
      significantly increased lower limit and upper limit values and has a 
      significantly widened range compared with the ex vivo method. When APTT values 
      are compared with both methods, more samples are underheparinized with the in 
      vitro curve method. The in vitro method is not a valid method to determine an 
      accurate therapeutic heparin range.
FAU - Gausman, Jana N
AU  - Gausman JN
AD  - Oklahoma City VA Medical Center, 921 13th St., Oklahoma City, OK 73104, USA.
FAU - Marlar, Richard A
AU  - Marlar RA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Anticoagulants)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Anticoagulants/*blood/*therapeutic use
MH  - Heparin/*blood/*therapeutic use
MH  - Humans
MH  - In Vitro Techniques
MH  - Partial Thromboplastin Time/*methods/*standards
MH  - Reproducibility of Results
EDAT- 2011/05/17 06:00
MHDA- 2011/07/13 06:00
CRDT- 2011/05/17 06:00
PHST- 2011/05/17 06:00 [entrez]
PHST- 2011/05/17 06:00 [pubmed]
PHST- 2011/07/13 06:00 [medline]
AID - 135/6/870 [pii]
AID - 10.1309/AJCP60ZGXCJKRMJO [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2011 Jun;135(6):870-6. doi: 10.1309/AJCP60ZGXCJKRMJO.