PMID- 21571964
OWN - NLM
STAT- MEDLINE
DCOM- 20110712
LR  - 20220316
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 135
IP  - 6
DP  - 2011 Jun
TI  - Diagnostic yield of bone marrow and peripheral blood FISH panel testing in 
      clinically suspected myelodysplastic syndromes and/or acute myeloid leukemia: a 
      prospective analysis of 433 cases.
PG  - 915-20
LID - 10.1309/AJCPW10YBRMWSWYE [doi]
AB  - It is unclear how often and in what setting fluorescence in situ hybridization 
      (FISH) panels for myelodysplastic syndrome (MDS)/acute myeloid leukemia (AML) 
      provide additional information over metaphase cytogenetics alone. Furthermore, 
      the usefulness of peripheral blood vs bone marrow FISH has also not been directly 
      compared. We prospectively compared metaphase cytogenetics and FISH for -5/5q-, 
      -7/7q-, +8, and 20q- in 433 cases of suspected MDS/AML. FISH testing was abnormal 
      in 6 (14%) of 43 and 10 (19%) of 54 cases with fewer than 20 normal metaphases or 
      no growth, respectively. FISH was only rarely abnormal in cases with 20 normal 
      metaphases obtained (6/222 [2.7%]). Comparison of peripheral blood and bone 
      marrow results in 48 cases showed abnormal peripheral blood FISH results in 18 
      (69%) of 26 cases with abnormal bone marrow FISH results and in 5 (23%) of 22 
      cases with normal bone marrow FISH results. These findings, the largest published 
      comparison of FISH vs metaphase cytogenetics in MDS/AML, provide a rational 
      strategy for FISH testing in peripheral blood and bone marrow.
FAU - Coleman, Joshua F
AU  - Coleman JF
AD  - Dept. of Clinical Pathology, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 
      44195, USA.
FAU - Theil, Karl S
AU  - Theil KS
FAU - Tubbs, Raymond R
AU  - Tubbs RR
FAU - Cook, James R
AU  - Cook JR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
SB  - IM
MH  - Bone Marrow/*metabolism
MH  - Cytogenetic Analysis
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myeloid, Acute/blood/*diagnosis/*metabolism
MH  - Metaphase
MH  - Myelodysplastic Syndromes/blood/*diagnosis/*metabolism
MH  - Prospective Studies
EDAT- 2011/05/17 06:00
MHDA- 2011/07/13 06:00
CRDT- 2011/05/17 06:00
PHST- 2011/05/17 06:00 [entrez]
PHST- 2011/05/17 06:00 [pubmed]
PHST- 2011/07/13 06:00 [medline]
AID - 135/6/915 [pii]
AID - 10.1309/AJCPW10YBRMWSWYE [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2011 Jun;135(6):915-20. doi: 10.1309/AJCPW10YBRMWSWYE.