PMID- 21573347
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20190606
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 3
IP  - 2
DP  - 1993 Aug
TI  - Use of fluorescence in-situ hybridization (fish) for the estimation of the 
      aberrant cell clone in leukemias with trisomy-8 or monosomy-7 detected by 
      karyotyping.
PG  - 191-5
AB  - The sizes of leukemic cell clones with hypersomies of the chromosome #8 or 
      monosomy #7, which primarily were detected by classical karyotyping, were 
      estimated by fluorescence in situ hybridization (FISH) with centromeric DNA 
      probes in interphase cell populations of the bone marrow of 31 leukemia patients. 
      Particularly, the data obtained by both routes of analysis were compared 
      quantitatively. As most prominent result, all aberrations found by classical 
      karyotyping were redetected by interphase cytogenetics, but additional aberrant 
      clones could be observed among the interphase cell populations. The frequencies 
      of the cell clones with hypersomies #8, in general, were higher in metaphase 
      than' in interphase, and, vice versa, cells monosomic for #7, in the majority of 
      cases, were found to be more frequent in interphase than in metaphase. These data 
      support the idea that metaphase data per se may not sufficiently reflect the 
      actual portions of aneuploid cell clones in the whole leukemic cell population. 
      This may be of practical importance in diagnostic and prognostic respects but 
      also for the choice of specific therapies.
FAU - Gebhart, E
AU  - Gebhart E
FAU - Trautmann, U
AU  - Trautmann U
FAU - Reichardt, S
AU  - Reichardt S
FAU - Liehr, T
AU  - Liehr T
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
EDAT- 1993/08/01 00:00
MHDA- 1993/08/01 00:01
CRDT- 2011/05/17 06:00
PHST- 2011/05/17 06:00 [entrez]
PHST- 1993/08/01 00:00 [pubmed]
PHST- 1993/08/01 00:01 [medline]
AID - 10.3892/ijo.3.2.191 [doi]
PST - ppublish
SO  - Int J Oncol. 1993 Aug;3(2):191-5. doi: 10.3892/ijo.3.2.191.