PMID- 21574396
OWN - NLM
STAT- MEDLINE
DCOM- 20120628
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 1
DP  - 2011 Jan
TI  - [Enantiomeric separation of propranolol by normal phase chiral liquid 
      chromatography coupled, with tandem mass spectrometry].
PG  - 26-30
AB  - A rapid and sensitive method was developed and validated using a normal phase 
      liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) for 
      determination of propranolol enantiomers in pharmaceuticals. Sample preparation 
      involved a single extraction step by the addition of methanol. Separation of 
      propranolol enantiomers was achieved on a Chiralcel OD-H chiral column using a 
      mobile phase consisting of n-hexane-ethanol-ammonia (70:30:0.4, v/v/v), and the 
      flow rate was 0.40 mL/min for 20 min. The analyte was monitored by tandem mass 
      spectrometry with electrospray positive ionization in multiple reaction 
      monitoring (MRM) mode, using the transitions of m/z 260.2 --> 116.0. Propranolol 
      enantiomers can be completely separated. The linear range was 2.5-1000 microg/L, 
      and the limit of quantification (LOQ) was 2.5 microg/L. The values for within day 
      and between day precisions and accuracies were well within the generally accepted 
      criteria for analytical methods. The relative standard deviations (RSDs) were 
      less than 2.64%, and the recoveries of the two enantiomers were 99.08%-102.58% 
      and 100.21%-103.16%, respectively. The separation method is accurate, convenient, 
      reliable, efficient, and can be subsequently used for quality control of 
      propranolol enantiomers in pharmaceuticals.
FAU - Zhang, Juanhong
AU  - Zhang J
AD  - Base for Clinical Pharmacology, Lanzhou General Hospital of Lanzhou Military 
      Region, Lanzhou 730050, China.
FAU - Wang, Rong
AU  - Wang R
FAU - Xie, Hua
AU  - Xie H
FAU - Meng, Xiandong
AU  - Meng X
FAU - Jia, Zhengping
AU  - Jia Z
FAU - Ma, Jun
AU  - Ma J
FAU - Zhang, Junli
AU  - Zhang J
FAU - Wang, Juan
AU  - Wang J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 9Y8NXQ24VQ (Propranolol)
SB  - IM
MH  - Chromatography, Liquid/*methods
MH  - Propranolol/*chemistry
MH  - Stereoisomerism
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2011/05/18 06:00
MHDA- 2012/06/29 06:00
CRDT- 2011/05/18 06:00
PHST- 2011/05/18 06:00 [entrez]
PHST- 2011/05/18 06:00 [pubmed]
PHST- 2012/06/29 06:00 [medline]
AID - 10.3724/sp.j.1123.2011.00026 [doi]
PST - ppublish
SO  - Se Pu. 2011 Jan;29(1):26-30. doi: 10.3724/sp.j.1123.2011.00026.