PMID- 21575390
OWN - NLM
STAT- MEDLINE
DCOM- 20111017
LR  - 20221207
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 40
IP  - 3
DP  - 2011 Mar
TI  - [Detection of TERC gene amplification by fluorescence in-situ hybridization in 
      cervical intraepithelial lesions].
PG  - 182-6
AB  - OBJECTIVE: To explore the feasibility and practical value of fluorescence in situ 
      hybridization (FISH) detection of TERC gene amplification in cervical 
      intraepithelial lesions (CIN) and squamous cell carcinoma (SCC). METHODS: Tissue 
      microarray was constructed to cover 150 cases of various cervical conditions, 
      including 24 cases of normal cervical mucosa, 78 cases of CINs (CINI, 25 cases; 
      CINII, 21 cases and CINIII, 32 cases) and 48 cases of SCC. FISH was used to 
      detect TERC gene amplification. RESULTS: TERC gene amplification was detected in 
      8% (2/25) CINI, 47.6% (10/21) CINII, 71.9% (23/32) CINIII and 87.5% (42/48) SCC. 
      There were significant differences among these groups (P < 0.05). The 
      amplification rates of TERC gene in SCC, CINIII and CINII were significantly 
      higher than those of normal cervical epithelium and CINI (P < 0.05). Significant 
      differences were also observed among CINI and CINII, CINIII and SCC (P < 0.05), 
      and between CINII and SCC (P < 0.05). There were no significant differences 
      between normal cervical epithelium and CINI, CINII and CIN III, and between 
      CINIII and SCC (P > 0.05). FISH detection of amplification of TERC gene in CINI 
      and CINII-III demonstrated the following statistics: sensitivity of 62.3%, 
      specificity of 92.0%, accuracy of 71.8%, positive and negative predictive values 
      of 94.3% and 53.5%, respectively. CONCLUSIONS: FISH detection is a reliable 
      method in detecting TERC gene amplification using paraffin tissue sections. When 
      histological evaluation becomes difficult, TERC amplification detectable by FISH 
      may offer a diagnostic distinction of CINI from CINII. Moreover, TERC 
      amplification may be used as a biomarker in predicting CIN progression to 
      invasive cancer.
FAU - YUAN, Yan-long
AU  - YUAN YL
AD  - Department of Pathology, Hebei General Hospital, Shijiazhuang 050051, China.
FAU - HE, Chun-nian
AU  - HE CN
FAU - XU, Ming-tang
AU  - XU MT
FAU - XU, Cui-qing
AU  - XU CQ
FAU - SUN, Yu-ning
AU  - SUN YN
FAU - ZHAO, Huan-fen
AU  - ZHAO HF
FAU - CHEN, Chen
AU  - CHEN C
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Adenoma/diagnosis/genetics
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/analysis
MH  - Carcinoma, Squamous Cell/diagnosis/*genetics
MH  - Disease Progression
MH  - Female
MH  - *Gene Amplification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - RNA/*genetics
MH  - Sensitivity and Specificity
MH  - Telomerase/*genetics
MH  - Uterine Cervical Neoplasms/diagnosis/*genetics
MH  - Young Adult
MH  - Uterine Cervical Dysplasia/diagnosis/*genetics
EDAT- 2011/05/18 06:00
MHDA- 2011/10/18 06:00
CRDT- 2011/05/18 06:00
PHST- 2011/05/18 06:00 [entrez]
PHST- 2011/05/18 06:00 [pubmed]
PHST- 2011/10/18 06:00 [medline]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2011 Mar;40(3):182-6.