PMID- 21575519
OWN - NLM
STAT- MEDLINE
DCOM- 20120426
LR  - 20110517
IS  - 0253-3766 (Print)
IS  - 0253-3766 (Linking)
VI  - 33
IP  - 3
DP  - 2011 Mar
TI  - [Correlation of EphA2 protein expression with clinicopathological characteristics 
      and prognosis in laryngeal squamous cell carcinoma].
PG  - 197-201
AB  - OBJECTIVE: To evaluate the expression of EphA2 protein in tissue specimens and 
      cell lines of laryngeal squamous cell carcinoma (LSCC), and to further study the 
      correlation of EphA2 protein expression with clinicopathological characteristics 
      and prognosis in LSCC. METHODS: Western blot was applied to assess the EphA2 
      protein expression in LSCC cell line Hep-2 cells and the head and neck 
      immortalized epithelial cell line NP-69 cells. Immunohistochemical staining was 
      performed on paraffin sections of 88 cases of LSCC specimens and 16 cases of 
      adjcent normal tissue samples to investigate the EphA2 protein expression, and to 
      futher elucidate its correlation with clinicopathological characteristics. 
      RESULTS: Compared with the NP-69 cells, EphA2 expression in LSCC cell line Hep-2 
      cells was upregulated. The positive rates of EphA2 expression in LSCC and adjcent 
      normal tissues samples were 80.7% and 43.8%, respectively, with a significant 
      difference between the two groups (P < 0.001). EphA2 overexpresion was closely 
      correlated with clinical stage (I + II/III + IV, P = 0.005), metastasis (P = 
      0.025) and recurrence (P = 0.021) in LSCC. Furthermore, patients with EphA2 
      overexpression had poorer tumor-free survival and 5-year overall survival 
      compared with that in patients with low EphA2 expression (33.3% vs. 63.2%, P = 
      0.003; 46.7% vs. 81.6%, P = 0.002). EphA2 expression combined with clinical stage 
      provided a better predictive value in prognosis. Univariate and multivariate Cox 
      regression analysis revealed that EphA2 expression is an independent prognostic 
      factor for patients with LSCC (P = 0.019). CONCLUSIONS: The results of this study 
      demonstrate that EphA2 protein expression is significantly increased in LSCC 
      tissues and cell lines, and EphA2 protein overexpression is associated with tumor 
      recurrence, metastasis and poorer prognosis in LSCC patients. These results 
      suggest that EphA2 may play a critical role in the initiation and progression of 
      LSCC, implicating EphA2 as a valuable marker for the prediction of recurrence, 
      metastasis and prognosis in LSCC.
FAU - Liu, Yong
AU  - Liu Y
AD  - Department of Otorhinolaryngology, Xiangya Hospital, Central South University, 
      Changsha 410008, China.
FAU - Zhang, Xin
AU  - Zhang X
FAU - Qiu, Yuan-zheng
AU  - Qiu YZ
FAU - Huang, Dong-hai
AU  - Huang DH
FAU - Zhou, Xiao-juan
AU  - Zhou XJ
FAU - Tan, Ping-qing
AU  - Tan PQ
FAU - Cai, Geng-ming
AU  - Cai GM
FAU - Dai, Yao-zhang
AU  - Dai YZ
FAU - Yu, Chang-yun
AU  - Yu CY
FAU - Xiao, Jian-yun
AU  - Xiao JY
FAU - Tian, Yong-quan
AU  - Tian YQ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Zhong Liu Za Zhi
JT  - Zhonghua zhong liu za zhi [Chinese journal of oncology]
JID - 7910681
RN  - EC 2.7.10.1 (Receptor, EphA2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/*metabolism/*pathology/surgery
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Disease-Free Survival
MH  - Epithelial Cells/metabolism
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - Laryngeal Neoplasms/*metabolism/*pathology/surgery
MH  - Lymphatic Metastasis
MH  - Male
MH  - Middle Aged
MH  - Neck
MH  - Neoplasm Recurrence, Local
MH  - Neoplasm Staging
MH  - Proportional Hazards Models
MH  - Receptor, EphA2/*metabolism
MH  - Survival Rate
EDAT- 2011/05/18 06:00
MHDA- 2012/04/27 06:00
CRDT- 2011/05/18 06:00
PHST- 2011/05/18 06:00 [entrez]
PHST- 2011/05/18 06:00 [pubmed]
PHST- 2012/04/27 06:00 [medline]
PST - ppublish
SO  - Zhonghua Zhong Liu Za Zhi. 2011 Mar;33(3):197-201.