PMID- 21577096
OWN - NLM
STAT- MEDLINE
DCOM- 20111223
LR  - 20181201
IS  - 1473-5733 (Electronic)
IS  - 0957-5235 (Linking)
VI  - 22
IP  - 5
DP  - 2011 Jul
TI  - Differential coagulation inhibitory effect of fondaparinux, enoxaparin and 
      unfractionated heparin in cell models of thrombin generation.
PG  - 369-73
LID - 10.1097/MBC.0b013e328344f7d0 [doi]
AB  - Anticoagulants, including unfractionated heparin (UFH), enoxaparin and 
      fondaparinux, are approved drugs in acute coronary syndrome (ACS). Monocytes and 
      monocyte-derived microparticles (MMPs) play an important procoagulant role in ACS 
      by expressing high tissue factor (TF) levels, which in turn triggers thrombin 
      generation. The objective of our study is to compare the in-vitro inhibitory 
      effect of UFH, enoxaparin and fondaparinux in monocytes and MMP models. 
      Human-elutriated monocytes were activated for 5 and 18 h by lipopolysaccharide to 
      obtain activated monocytes (ac-M) or MMPs, respectively. Thrombin generation 
      inhibition was assessed using ac-M or MMPs mixed with platelet-poor plasma 
      containing increased concentrations of anticoagulants. Thrombin generation 
      inhibition was dose-dependent with a differential effect according to the drug: 
      the highest for UFH, the lowest for fondaparinux. Rate index was the most 
      sensitive parameter. For fondaparinux, its IC50 values (anti-Xa IU/ml) were 
      0.59+/-0.05 for ac-M and 0.17+/-0.03 for MMPs. For enoxaparin, rate index IC50 values 
      were 0.27+/-0.03 for ac-M and 0.19+/-0.02 for MMPs. Our data support the notion that 
      cell-induced thrombin generation assay may be a reliable alternative to anti-Xa 
      assessment in determining patient anticoagulation level.
FAU - Ben-Hadj-Khalifa, Sonia
AU  - Ben-Hadj-Khalifa S
AD  - Unite de Recherche des Maladies Hematologiques et Auto-Immunes, Monastir, 
      Tunisia.
FAU - Hezard, Nathalie
AU  - Hezard N
FAU - Almawi, Wassim Y
AU  - Almawi WY
FAU - Remy, Marie G
AU  - Remy MG
FAU - Florent, Bernadette
AU  - Florent B
FAU - Mahjoub, Touhami
AU  - Mahjoub T
FAU - Nguyen, Philippe
AU  - Nguyen P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Blood Coagul Fibrinolysis
JT  - Blood coagulation & fibrinolysis : an international journal in haemostasis and 
      thrombosis
JID - 9102551
RN  - 0 (Anticoagulants)
RN  - 0 (Enoxaparin)
RN  - 0 (Polysaccharides)
RN  - 9005-49-6 (Heparin)
RN  - EC 3.4.21.5 (Thrombin)
RN  - J177FOW5JL (Fondaparinux)
SB  - IM
MH  - Anticoagulants/*pharmacology
MH  - Blood Coagulation/*drug effects
MH  - Cell-Derived Microparticles/drug effects
MH  - Cells, Cultured
MH  - Enoxaparin/pharmacology
MH  - Fondaparinux
MH  - Heparin/pharmacology
MH  - Humans
MH  - Inhibitory Concentration 50
MH  - Models, Biological
MH  - Monocytes/cytology/*drug effects
MH  - Polysaccharides/pharmacology
MH  - Thrombin/*antagonists & inhibitors/biosynthesis
EDAT- 2011/05/18 06:00
MHDA- 2011/12/24 06:00
CRDT- 2011/05/18 06:00
PHST- 2011/05/18 06:00 [entrez]
PHST- 2011/05/18 06:00 [pubmed]
PHST- 2011/12/24 06:00 [medline]
AID - 10.1097/MBC.0b013e328344f7d0 [doi]
PST - ppublish
SO  - Blood Coagul Fibrinolysis. 2011 Jul;22(5):369-73. doi: 
      10.1097/MBC.0b013e328344f7d0.