PMID- 215778
OWN - NLM
STAT- MEDLINE
DCOM- 19790328
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 28
IP  - 3
DP  - 1978 Dec
TI  - Detection of mouse mammary tumor virus RNA in BALB/c tumor cell lines of nonviral 
      etiologies.
PG  - 743-52
AB  - A complementary DNA (cDNA) probe to mouse mammary tumor virus (MMTV) RNA was 
      synthesized using calf thymus DNA oligonucleotides as a random primer. This probe 
      was then used to study the expression of MMTV RNA in cell lines from BALB/c 
      tumors induced in vivo either spontaneously or in response to viral, chemical, or 
      hormonal stimuli. The cDNA had a length of approximately 400 to 500 nucleotides 
      and specifically hybridized to MMTV RNA and BALB/c lactating mammary gland RNA, 
      but not to Moloney leukemia virus RNA. Calf thymus DNA-primed cDNA could protect 
      50% of iodinated MMTV RNA from S1 nuclease digestion at cDNA-RNA ratios of 1:1 
      and 90% of labeled viral RNA at ratios of 10:1. Thermal denaturation of MMTV 
      RNA-cDNA hybrids yielded a T(m) of 88.5 degrees C, indicative of a 
      well-base-paired duplex. Screening of mouse mammary tumor cells for MMTV 
      sequences revealed that three out of five lines of BALB/c origin had undetectable 
      levels of viral RNA (<three molecules per cell) by RNA excess hybridization. Two 
      of the three "virus-negative" cell lines were derived from tumors induced by the 
      chemical carcinogen 7,12-dimethylbenz(alpha)anthracene, whereas the third tumor 
      occurred spontaneously. Two lines from tumors induced by either viral (mammary 
      tumor virus) or hormonal (17-beta-estradiol) stimulus contained between three and 
      nine molecules of MMTV RNA per cell by both RNA excess and cDNA excess 
      hybridization. Clonal derivatives of these tumor lines had levels of viral RNA 
      comparable to those of their parental lines. Therefore, it appears that the 
      presence of detectable MMTV RNA sequences is not a necessary requirement for the 
      maintenance of all murine mammary gland neoplasias.
FAU - Dudley, J P
AU  - Dudley JP
FAU - Butel, J S
AU  - Butel JS
FAU - Socher, S H
AU  - Socher SH
FAU - Rosen, J M
AU  - Rosen JM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (RNA, Viral)
SB  - IM
MH  - *Cell Line
MH  - Clone Cells
MH  - *Mammary Tumor Virus, Mouse
MH  - Neoplasms/*analysis
MH  - Neoplasms, Experimental/*analysis
MH  - Nucleic Acid Hybridization
MH  - RNA, Viral/*analysis
PMC - PMC525798
EDAT- 1978/12/01 00:00
MHDA- 1978/12/01 00:01
PMCR- 1978/12/01
CRDT- 1978/12/01 00:00
PHST- 1978/12/01 00:00 [pubmed]
PHST- 1978/12/01 00:01 [medline]
PHST- 1978/12/01 00:00 [entrez]
PHST- 1978/12/01 00:00 [pmc-release]
AID - 10.1128/JVI.28.3.743-752.1978 [doi]
PST - ppublish
SO  - J Virol. 1978 Dec;28(3):743-52. doi: 10.1128/JVI.28.3.743-752.1978.