PMID- 2158150
OWN - NLM
STAT- MEDLINE
DCOM- 19900524
LR  - 20190727
IS  - 0049-3848 (Print)
IS  - 0049-3848 (Linking)
VI  - 57
IP  - 4
DP  - 1990 Feb 15
TI  - Heparin enhances thrombin-stimulated prostaglandin I2 production by cultured 
      endothelial cells.
PG  - 481-8
AB  - Endothelial cells from human umbilical vein were incubated in vitro with thrombin 
      in the absence or presence of unfractionated heparin (UFH), low molecular weight 
      heparin (LMWH) or dermatan sulfate (DS) to clarify the effect of these 
      glycosaminoglycans on thrombin-stimulated prostaglandin I2 (PGI2) production. 
      Although DS did not affect the thrombin stimulation, UFH and LMWH each enhanced 
      it about 1.5-fold. Pretreatment of cells with either UFH or LMWH also enhanced 
      the thrombin stimulation. In addition, UFH and LMWH each enhanced PGI2 production 
      stimulated by calcium ionophore A23187, but did not affect that stimulated by 
      arachidonic acid. From these results, it was suggested that UFH and LMWH might 
      cause a physical change of cell membrane, which would caused an easier liberation 
      of arachidonic acid by phospholipases.
FAU - Itoh, F
AU  - Itoh F
AD  - Department of Clinical Laboratory Medicine, Faculty of Medicine, Toyama Medical 
      and Pharmaceutical University, Japan.
FAU - Kaji, T
AU  - Kaji T
FAU - Hayakawa, Y
AU  - Hayakawa Y
FAU - Oguma, Y
AU  - Oguma Y
FAU - Sakuragawa, N
AU  - Sakuragawa N
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 0 (Arachidonic Acids)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 24967-94-0 (Dermatan Sulfate)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 9005-49-6 (Heparin)
RN  - DCR9Z582X0 (Epoprostenol)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Arachidonic Acid
MH  - Arachidonic Acids/pharmacology
MH  - Calcimycin/pharmacology
MH  - Cells, Cultured
MH  - Dermatan Sulfate/pharmacology
MH  - Endothelium, Vascular/cytology/*drug effects
MH  - Epoprostenol/*biosynthesis
MH  - Heparin/*pharmacology
MH  - Heparin, Low-Molecular-Weight/pharmacology
MH  - Humans
MH  - Stimulation, Chemical
MH  - Thrombin/pharmacology
EDAT- 1990/02/15 00:00
MHDA- 1990/02/15 00:01
CRDT- 1990/02/15 00:00
PHST- 1990/02/15 00:00 [pubmed]
PHST- 1990/02/15 00:01 [medline]
PHST- 1990/02/15 00:00 [entrez]
AID - 10.1016/0049-3848(90)90065-k [doi]
PST - ppublish
SO  - Thromb Res. 1990 Feb 15;57(4):481-8. doi: 10.1016/0049-3848(90)90065-k.