PMID- 21584819
OWN - NLM
STAT- MEDLINE
DCOM- 20120711
LR  - 20211203
IS  - 1434-4726 (Electronic)
IS  - 0937-4477 (Print)
IS  - 0937-4477 (Linking)
VI  - 269
IP  - 2
DP  - 2012 Feb
TI  - Overexpression of Aurora-A promotes laryngeal cancer progression by enhancing 
      invasive ability and chromosomal instability.
PG  - 607-14
LID - 10.1007/s00405-011-1629-4 [doi]
AB  - The purpose of the study was to investigate the expression of Aurora-A in human 
      laryngeal squamous cell carcinoma (LSCC) and to explore the effects of Aurora-A 
      silencing on invasion and chromosomal instability in laryngeal cancer HEp-2 
      cells. The expression of Aurora-A mRNA and protein were studied using reverse 
      transcription-PCR and Western blot in LSCC tissues and corresponding normal 
      epithelium, respectively. In addition, the correlation between Aurora-A 
      expression and clinicopathologic characteristics was analyzed in LSCC patients. 
      Furthermore, HEp-2 cells were transfected with Aurora-A short hairpin RNA and the 
      effects of knockdown of Aurora-A on tumor invasion and chromosomal instability 
      were investigated. The results showed that expression of Aurora-A mRNA was 
      significantly upregulated in laryngeal tumor tissue compared with that in normal 
      tissue (P = 0.001), and overexpression of Aurora-A was found in 64.0% (16 of 25) 
      of the patients by Western blotting. Upregulation of Aurora-A mRNA was 
      significantly correlated with regional lymph node metastasis (P = 0.007) and 
      clinical stage III/IV (P = 0.022). Overexpression of Aurora-A was significantly 
      associated with lymph node metastasis (P = 0.027). Furthermore, disruption of 
      Aurora-A using RNA interference technique suppressed invasive ability and 
      chromosomal instability in HEp-2 cells. In conclusion, Aurora-A expression is 
      elevated in human LSCC and associated with regional lymph node metastasis and 
      late clinical stage. Overexpression of Aurora-A may contribute to LSCC 
      carcinogenesis and progression partially due to enhancement of invasion ability 
      and chromosomal instability.
FAU - Zhang, Hao
AU  - Zhang H
AD  - Department of Otolaryngology, Head and Neck Surgery, Eye Ear Nose and Throat 
      Hospital, Fudan University, 83 FenYang Road, Shanghai, 200031, China.
FAU - Chen, Xuehua
AU  - Chen X
FAU - Jin, Yuesheng
AU  - Jin Y
FAU - Liu, Bingya
AU  - Liu B
FAU - Zhou, Liang
AU  - Zhou L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110517
PL  - Germany
TA  - Eur Arch Otorhinolaryngol
JT  - European archives of oto-rhino-laryngology : official journal of the European 
      Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the 
      German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
JID - 9002937
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.1 (Aurora Kinases)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Aged
MH  - Aurora Kinases
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Chromosomal Instability/*genetics
MH  - Disease Progression
MH  - Female
MH  - Gene Silencing
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Larynx/pathology
MH  - Lymphatic Metastasis/genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Invasiveness/genetics/pathology
MH  - Neoplasm Staging
MH  - Protein Serine-Threonine Kinases/*genetics
MH  - RNA, Messenger/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Up-Regulation/genetics
PMC - PMC3259349
EDAT- 2011/05/18 06:00
MHDA- 2012/07/12 06:00
PMCR- 2011/05/17
CRDT- 2011/05/18 06:00
PHST- 2011/02/13 00:00 [received]
PHST- 2011/05/02 00:00 [accepted]
PHST- 2011/05/18 06:00 [entrez]
PHST- 2011/05/18 06:00 [pubmed]
PHST- 2012/07/12 06:00 [medline]
PHST- 2011/05/17 00:00 [pmc-release]
AID - 1629 [pii]
AID - 10.1007/s00405-011-1629-4 [doi]
PST - ppublish
SO  - Eur Arch Otorhinolaryngol. 2012 Feb;269(2):607-14. doi: 
      10.1007/s00405-011-1629-4. Epub 2011 May 17.