PMID- 21587207
OWN - NLM
STAT- MEDLINE
DCOM- 20110916
LR  - 20211020
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 30
IP  - 13
DP  - 2011 May 17
TI  - Id2 expression delineates differential checkpoints in the genetic program of 
      CD8alpha+ and CD103+ dendritic cell lineages.
PG  - 2690-704
LID - 10.1038/emboj.2011.163 [doi]
AB  - Dendritic cells (DCs) have critical roles in the induction of the adaptive immune 
      response. The transcription factors Id2, Batf3 and Irf-8 are required for many 
      aspects of murine DC differentiation including development of CD8alpha(+) and 
      CD103(+) DCs. How they regulate DC subset specification is not completely 
      understood. Using an Id2-GFP reporter system, we show that Id2 is broadly 
      expressed in all cDC subsets with the highest expression in CD103(+) and CD8alpha(+) 
      lineages. Notably, CD103(+) DCs were the only DC able to constitutively 
      cross-present cell-associated antigens in vitro. Irf-8 deficiency affected loss 
      of development of virtually all conventional DCs (cDCs) while Batf3 deficiency 
      resulted in the development of Sirp-alpha(-) DCs that had impaired survival. Exposure 
      to GM-CSF during differentiation induced expression of CD103 in Id2-GFP(+) DCs. 
      It did not restore cross-presenting capacity to Batf3(-/-) or 
      CD103(-)Sirp-alpha(-)DCs in vitro. Thus, Irf-8 and Batf3 regulate distinct stages in 
      DC differentiation during the development of cDCs. Genetic mapping DC subset 
      differentiation using Id2-GFP may have broad implications in understanding the 
      interplay of DC subsets during protective and pathological immune responses.
FAU - Jackson, Jacob T
AU  - Jackson JT
AD  - Molecular Immunology Division, The Walter and Eliza Hall Institute of Medical 
      Research, Melbourne, Victoria, Australia.
FAU - Hu, Yifang
AU  - Hu Y
FAU - Liu, Ruijie
AU  - Liu R
FAU - Masson, Frederick
AU  - Masson F
FAU - D'Amico, Angela
AU  - D'Amico A
FAU - Carotta, Sebastian
AU  - Carotta S
FAU - Xin, Annie
AU  - Xin A
FAU - Camilleri, Mary J
AU  - Camilleri MJ
FAU - Mount, Adele M
AU  - Mount AM
FAU - Kallies, Axel
AU  - Kallies A
FAU - Wu, Li
AU  - Wu L
FAU - Smyth, Gordon K
AU  - Smyth GK
FAU - Nutt, Stephen L
AU  - Nutt SL
FAU - Belz, Gabrielle T
AU  - Belz GT
LA  - eng
GR  - Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110517
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Antigens, CD)
RN  - 0 (CD8 Antigens)
RN  - 0 (CD8 antigen, alpha chain)
RN  - 0 (Idb2 protein, mouse)
RN  - 0 (Inhibitor of Differentiation Protein 2)
RN  - 0 (Integrin alpha Chains)
RN  - 0 (alpha E integrins)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*metabolism
MH  - CD8 Antigens/*metabolism
MH  - Cell Differentiation/genetics/physiology
MH  - Cell Lineage/*genetics
MH  - Cells, Cultured
MH  - Dendritic Cells/metabolism/*physiology
MH  - Gene Expression/physiology
MH  - Genes, cdc/physiology
MH  - Inhibitor of Differentiation Protein 2/*genetics/physiology
MH  - Integrin alpha Chains/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Models, Biological
PMC - PMC3155298
COIS- The authors declare that they have no conflict of interest.
EDAT- 2011/05/19 06:00
MHDA- 2011/09/17 06:00
PMCR- 2011/05/17
CRDT- 2011/05/19 06:00
PHST- 2011/02/24 00:00 [received]
PHST- 2011/04/28 00:00 [accepted]
PHST- 2011/05/19 06:00 [entrez]
PHST- 2011/05/19 06:00 [pubmed]
PHST- 2011/09/17 06:00 [medline]
PHST- 2011/05/17 00:00 [pmc-release]
AID - emboj2011163 [pii]
AID - 10.1038/emboj.2011.163 [doi]
PST - epublish
SO  - EMBO J. 2011 May 17;30(13):2690-704. doi: 10.1038/emboj.2011.163.