PMID- 21590355
OWN - NLM
STAT- MEDLINE
DCOM- 20120224
LR  - 20220330
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 357
IP  - 1-2
DP  - 2011 Nov
TI  - Effects of stable knockdown of Aurora kinase A on proliferation, migration, 
      chromosomal instability, and expression of focal adhesion kinase and matrix 
      metalloproteinase-2 in HEp-2 cells.
PG  - 95-106
LID - 10.1007/s11010-011-0879-1 [doi]
AB  - Overexpression of Aurora kinase A (AURKA) is frequently observed in various 
      cancers, including laryngeal squamous cell carcinoma (LSCC). We investigated the 
      effects of knockdown of AURKA on laryngeal cancer HEp-2 cells both in vitro and 
      in vivo. A plasmid containing short hairpin (sh)RNA against AURKA was constructed 
      and transfected into HEp-2. Measurements included the CCK-8 assay for viability 
      and proliferation, flow cytometry for apoptosis and effects on the mitotic 
      checkpoint, a trans-well assay for migration, immunofluorescence for assessment 
      of genomic instability, and western blotting for protein expression. AURKA 
      knockdown inhibited proliferation, migration, and colony formation in vitro and 
      tumorigenicity in vivo. The knockdown induced the accumulation of cells in G2-M 
      phase and eventual apoptosis. Knockdown of AURKA caused delayed entry into 
      mitosis after treatment with nocodazole, reduced chromosomal instability, and 
      decreased expression of focal adhesion kinase (FAK), phosphorylated FAK, and 
      matrix metalloproteinase-2 (MMP-2), key regulators in cell adhesion and invasion. 
      Knockdown of AURKA inhibits the growth and invasiveness of this LSCC cell line 
      both in vitro and in vivo. These effects may partially result from the reduced 
      expression of FAK and MMP-2. Knockdown of AURKA expression may represent a 
      promising therapeutic strategy for the treatment of LSCC.
FAU - Zhang, Hao
AU  - Zhang H
AD  - Department of Otolaryngology, Head and Neck Surgery, Eye Ear Nose and Throat 
      Hospital, Fudan University, Shanghai 200031, China.
FAU - Chen, Xuehua
AU  - Chen X
FAU - Liu, Bingya
AU  - Liu B
FAU - Zhou, Liang
AU  - Zhou L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110518
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - EC 2.7.10.2 (Focal Adhesion Protein-Tyrosine Kinases)
RN  - EC 2.7.11.1 (AURKA protein, human)
RN  - EC 2.7.11.1 (Aurka protein, mouse)
RN  - EC 2.7.11.1 (Aurora Kinase A)
RN  - EC 2.7.11.1 (Aurora Kinases)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Animals
MH  - Aurora Kinase A
MH  - Aurora Kinases
MH  - Carcinogenicity Tests
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation
MH  - Chromosomal Instability/genetics
MH  - Focal Adhesion Protein-Tyrosine Kinases/genetics/*metabolism
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Laryngeal Neoplasms/metabolism
MH  - Matrix Metalloproteinase 2/genetics/*metabolism
MH  - Mice
MH  - Mice, Nude
MH  - Protein Serine-Threonine Kinases/*genetics/*metabolism
MH  - RNA Interference
EDAT- 2011/05/19 06:00
MHDA- 2012/03/01 06:00
CRDT- 2011/05/19 06:00
PHST- 2011/02/18 00:00 [received]
PHST- 2011/05/06 00:00 [accepted]
PHST- 2011/05/19 06:00 [entrez]
PHST- 2011/05/19 06:00 [pubmed]
PHST- 2012/03/01 06:00 [medline]
AID - 10.1007/s11010-011-0879-1 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2011 Nov;357(1-2):95-106. doi: 10.1007/s11010-011-0879-1. Epub 
      2011 May 18.