PMID- 21593273
OWN - NLM
STAT- MEDLINE
DCOM- 20111011
LR  - 20200228
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 92
IP  - Pt 9
DP  - 2011 Sep
TI  - The helper component-proteinase of the Zucchini yellow mosaic virus inhibits the 
      Hua Enhancer 1 methyltransferase activity in vitro.
PG  - 2222-2226
LID - 10.1099/vir.0.031534-0 [doi]
AB  - The helper component-proteinase (HC-Pro) is a multifunctional protein found among 
      potyviruses. With respect to its silencing suppressor function, small RNA binding 
      appears to be the major activity of HC-Pro. HC-Pro could also exhibit other 
      suppressor activities. HC-Pro may inhibit the Hua Enhancer 1 (HEN1) activity. 
      There is indirect evidence showing that either transient or stable expression of 
      HC-Pro in plants results in an increase of non-methylated small RNAs. Here, we 
      demonstrated that recombinant Zucchini yellow mosaic virus (ZYMV) HC-Pro 
      inhibited the methyltransferase activity of HEN1 in vitro. Moreover, we found 
      that the HC-Pro(FINK) mutant, which has lost small RNA-binding activity, 
      inhibited HEN1 activity, while the truncated proteins and total soluble bacterial 
      proteins did not. Using the ELISA-binding assay, we provided evidence that the 
      HC-Pro(FRNK) wild-type and HC-Pro(FINK) both bound to HEN1, with HC-Pro(FRNK) 
      binding stronger than HC-Pro(FINK). Motif mapping analysis revealed that the 
      amino acids located between positions 139 and 320 of ZYMV HC-Pro were associated 
      with HEN1 interaction.
FAU - Jamous, Rana M
AU  - Jamous RM
AD  - RLP-AgroScience GmbH, AlPlanta-Institute for Plant Research, Breitenweg 71, 67435 
      Neustadt, Germany.
AD  - Biodiversity and Environmental Research Center-BERC, Til-Nablus, PO Box 696, 
      Palestinian Authority.
FAU - Boonrod, Kajohn
AU  - Boonrod K
AD  - RLP-AgroScience GmbH, AlPlanta-Institute for Plant Research, Breitenweg 71, 67435 
      Neustadt, Germany.
FAU - Fuellgrabe, Marc W
AU  - Fuellgrabe MW
AD  - RLP-AgroScience GmbH, AlPlanta-Institute for Plant Research, Breitenweg 71, 67435 
      Neustadt, Germany.
FAU - Ali-Shtayeh, Mohammed S
AU  - Ali-Shtayeh MS
AD  - Biodiversity and Environmental Research Center-BERC, Til-Nablus, PO Box 696, 
      Palestinian Authority.
FAU - Krczal, Gabi
AU  - Krczal G
AD  - RLP-AgroScience GmbH, AlPlanta-Institute for Plant Research, Breitenweg 71, 67435 
      Neustadt, Germany.
FAU - Wassenegger, Michael
AU  - Wassenegger M
AD  - Centre for Organismal Studies (COS), University of Heidelberg, Im Neuenheimer 
      Feld 360, 69120 Heidelberg, Germany.
AD  - RLP-AgroScience GmbH, AlPlanta-Institute for Plant Research, Breitenweg 71, 67435 
      Neustadt, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110518
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (HEN1 protein, Arabidopsis)
RN  - 0 (Viral Proteins)
RN  - EC 2.1.1.- (Methyltransferases)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.- (HC-Pro protein, potyvirus)
SB  - IM
MH  - Arabidopsis Proteins/*antagonists & inhibitors
MH  - Cysteine Endopeptidases/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Host-Pathogen Interactions
MH  - Methyltransferases/*antagonists & inhibitors
MH  - Mutation, Missense
MH  - Potyvirus/*enzymology/*pathogenicity
MH  - Protein Binding
MH  - Protein Interaction Mapping
MH  - Sequence Deletion
MH  - Viral Proteins/*metabolism
EDAT- 2011/05/20 06:00
MHDA- 2011/10/12 06:00
CRDT- 2011/05/20 06:00
PHST- 2011/05/20 06:00 [entrez]
PHST- 2011/05/20 06:00 [pubmed]
PHST- 2011/10/12 06:00 [medline]
AID - 10.1099/vir.0.031534-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2011 Sep;92(Pt 9):2222-2226. doi: 10.1099/vir.0.031534-0. Epub 2011 
      May 18.