PMID- 2159345
OWN - NLM
STAT- MEDLINE
DCOM- 19900614
LR  - 20190609
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1052
IP  - 2
DP  - 1990 May 2
TI  - Human immunoglobulin G potentiates superoxide production induced by chemotactic 
      peptides and causes degranulation in isolated human neutrophils.
PG  - 243-7
AB  - Neutrophils are major cellular mediators of host defense and inflammation. They 
      can be activated to produce superoxide and to release the contents of their 
      granules to the extracellular space. We observed that monomeric human 
      immunoglobulin G (IgG) sensitizes these cells to the chemotactic peptide 
      N-formylmethionylleucylphenylalanine (fMLP). In cells submaximally stimulated by 
      fMLP this enhancement was especially prominent. With saturating fMLP 
      concentrations, the rate of O2- production was still about twice that in the 
      control. No synergy with other activators (phorbol myristate acetate, 
      concanavalin A) was observed. Binding of fMLP to the cells was decreased by IgG, 
      resembling the effect of cytochalasin B. IgG did not induce O2- production on its 
      own, but it stimulated degranulation of the neutrophils.
FAU - Aaku, E
AU  - Aaku E
AD  - Department of Medical Chemisty, University of Helsinki, Finland.
FAU - Sorsa, T
AU  - Sorsa T
FAU - Wilkstrom, M
AU  - Wilkstrom M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Immunoglobulin G)
RN  - 11028-71-0 (Concanavalin A)
RN  - 11062-77-4 (Superoxides)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 3.4.- (Cathepsins)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.20 (CTSG protein, human)
RN  - EC 3.4.21.20 (Cathepsin G)
RN  - EC 3.4.24.3 (Microbial Collagenase)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/metabolism
MH  - Cathepsin G
MH  - Cathepsins/metabolism
MH  - *Cell Degranulation
MH  - Concanavalin A/pharmacology
MH  - Drug Synergism
MH  - Humans
MH  - Immunoglobulin G/*physiology
MH  - In Vitro Techniques
MH  - Microbial Collagenase/metabolism
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacokinetics
MH  - Neutrophils/drug effects/*physiology
MH  - Serine Endopeptidases
MH  - Superoxides/*metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1990/05/02 00:00
MHDA- 1990/05/02 00:01
CRDT- 1990/05/02 00:00
PHST- 1990/05/02 00:00 [pubmed]
PHST- 1990/05/02 00:01 [medline]
PHST- 1990/05/02 00:00 [entrez]
AID - 0167-4889(90)90217-2 [pii]
AID - 10.1016/0167-4889(90)90217-2 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1990 May 2;1052(2):243-7. doi: 
      10.1016/0167-4889(90)90217-2.