PMID- 21595026
OWN - NLM
STAT- MEDLINE
DCOM- 20111010
LR  - 20191210
IS  - 1615-9314 (Electronic)
IS  - 1615-9306 (Linking)
VI  - 34
IP  - 13
DP  - 2011 Jul
TI  - QuEChERS-based extraction procedure for multifamily analysis of phytohormones in 
      vegetables by UHPLC-MS/MS.
PG  - 1517-24
LID - 10.1002/jssc.201100093 [doi]
AB  - A new method has been developed and validated for the simultaneous analysis of 
      different phytohormones (auxins, cytokinins and gibberellins) in vegetables. The 
      compounds were extracted using a QuEChERS-based method (acronym of quick, easy, 
      cheap, effective, rugged and safe). The separation and determination of the 
      selected phytohormones were carried out by ultra high-performance liquid 
      chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS), using 
      electrospray ionization source (ESI) in positive and negative ion modes. The 
      method was validated and mean recoveries were evaluated at three concentration 
      levels (50, 100 and 250 mug/kg), ranging from 75 to 110% at the three levels 
      assayed. Intra- and interday precisions, expressed as relative standard 
      deviations (RSDs), were lower than 20 and 25%, respectively. Limits of 
      quantification (LOQs) were equal or lower than 10 mug/kg. The developed procedure 
      was applied to seven courgette samples, and naphthylacetic acid, 
      naphthylacetamide and benzyladenine were found in some of the analysed samples.
CI  - Copyright (c) 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Flores, Maria Isabel Alarcon
AU  - Flores MI
AD  - Group Analytical Chemistry of Contaminants, Department of Analytical Chemistry, 
      Almeria University, Almeria, Spain.
FAU - Romero-Gonzalez, Roberto
AU  - Romero-Gonzalez R
FAU - Frenich, Antonia Garrido
AU  - Frenich AG
FAU - Vidal, Jose Luis Martinez
AU  - Vidal JL
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110519
PL  - Germany
TA  - J Sep Sci
JT  - Journal of separation science
JID - 101088554
RN  - 0 (Plant Extracts)
RN  - 0 (Plant Growth Regulators)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Plant Extracts/analysis/*isolation & purification
MH  - Plant Growth Regulators/analysis/*isolation & purification
MH  - Solid Phase Extraction/*methods
MH  - Tandem Mass Spectrometry/*methods
MH  - Vegetables/*chemistry
EDAT- 2011/05/20 06:00
MHDA- 2011/10/11 06:00
CRDT- 2011/05/20 06:00
PHST- 2011/02/02 00:00 [received]
PHST- 2011/03/21 00:00 [revised]
PHST- 2011/04/02 00:00 [accepted]
PHST- 2011/05/20 06:00 [entrez]
PHST- 2011/05/20 06:00 [pubmed]
PHST- 2011/10/11 06:00 [medline]
AID - 10.1002/jssc.201100093 [doi]
PST - ppublish
SO  - J Sep Sci. 2011 Jul;34(13):1517-24. doi: 10.1002/jssc.201100093. Epub 2011 May 
      19.