PMID- 21595617
OWN - NLM
STAT- MEDLINE
DCOM- 20111213
LR  - 20181023
IS  - 1300-7475 (Print)
IS  - 1300-7475 (Linking)
VI  - 21
IP  - 3
DP  - 2011 May-Jun
TI  - Quantifying the expression of VEGF using qRT-PCR, evaluation of MVD and their 
      correlation with clinicopathological factors in laryngeal squamous cell 
      carcinoma.
PG  - 137-44
LID - kbbihtisas.2011.015 [pii]
LID - 10.5606/kbbihtisas.2011.015 [doi]
AB  - OBJECTIVES: In this study we quantified the expression of vascular endothelial 
      growth factor (VEGF) using quantitative real-time polymerase chain reaction in 
      laryngeal squamous cell carcinoma (LSCC) tissues and evaluated the correlation 
      between the level of VEGF and microvessel density (MVD), and clinicopathological 
      factors. PATIENTS AND METHODS: Twenty-seven patients with LSCC undergoing total 
      or partial laryngectomy at the Ear, Nose, and Throat and Head and Neck Surgery 
      Department of the Izmir Tepecik Training and Research Hospital between September 
      2006 and July 2008. There was no VEGF expression in two patients that were 
      excluded from the study. Twenty-five patients (24 males, 1 female; mean age 61 
      years; range 43 to 82 years) were included in this study, but MVD levels of 10 
      patients could not be determined. RESULTS: As defined by the 2003 American Joint 
      Committee on Cancer (AJCC) TNM classification, seven patients (28%) were stage 1, 
      six patients (24%) were stage 2, four patients (16%) were stage 3, and eight 
      patients (32%) were stage 4. Thirteen patients (52%) had well-differentiated (G1) 
      tumors, and twelve had moderately differentiated tumors. Among the 15 patients 
      for whom the MVD was determined, the median value was 48, with a (range 13-78; 
      vessels / 3.76 mm2). Among the 25 patients for whom the VEGF level was 
      determined, the median value was 0.035 vessels / 3.76 mm2 (range 0.010-0.127). 
      CONCLUSION: We could not find a statistical correlation between 
      clinicopathological factors and either VEGF or MVD. Our study demonstrates that 
      VEGF is expressed by LSCC.
FAU - Kocdor, Pelin
AU  - Kocdor P
AD  - Department of Otolaryngology, Izmir Tepecik Training and Research Hospital, 
      Izmir, Turkey. pelinkocdor@yahoo.com
FAU - Coker, Isil
AU  - Coker I
FAU - Bayol, Umit
AU  - Bayol U
FAU - Altinel, Deniz
AU  - Altinel D
FAU - Purcu, Duygu Unuvar
AU  - Purcu DU
FAU - Kaptaner, Suat
AU  - Kaptaner S
FAU - Yigitbasi, Orhan Gazi
AU  - Yigitbasi OG
LA  - eng
PT  - Journal Article
PL  - Turkey
TA  - Kulak Burun Bogaz Ihtis Derg
JT  - Kulak burun bogaz ihtisas dergisi : KBB = Journal of ear, nose, and throat
JID - 100899230
RN  - 0 (Actins)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Actins/analysis/genetics
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/blood supply/*metabolism/pathology
MH  - DNA, Complementary/analysis
MH  - DNA, Neoplasm/analysis
MH  - Double-Blind Method
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/blood supply/*metabolism/pathology
MH  - Laryngectomy
MH  - Lymphatic Metastasis
MH  - Male
MH  - Microvessels/growth & development
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Prospective Studies
MH  - Real-Time Polymerase Chain Reaction/methods
MH  - Turkey
MH  - Vascular Endothelial Growth Factor A/genetics/*metabolism
EDAT- 2011/05/21 06:00
MHDA- 2011/12/14 06:00
CRDT- 2011/05/21 06:00
PHST- 2011/05/21 06:00 [entrez]
PHST- 2011/05/21 06:00 [pubmed]
PHST- 2011/12/14 06:00 [medline]
AID - kbbihtisas.2011.015 [pii]
AID - 10.5606/kbbihtisas.2011.015 [doi]
PST - ppublish
SO  - Kulak Burun Bogaz Ihtis Derg. 2011 May-Jun;21(3):137-44. doi: 
      10.5606/kbbihtisas.2011.015.