PMID- 21596017
OWN - NLM
STAT- MEDLINE
DCOM- 20110815
LR  - 20211020
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 409
IP  - 3
DP  - 2011 Jun 10
TI  - Sialyl Lewisx-dependent binding of human monocyte-derived dendritic cells to 
      selectins.
PG  - 459-64
LID - 10.1016/j.bbrc.2011.05.026 [doi]
AB  - The limited efficacy of monocyte-derived dendritic cell (mo-DC)-based vaccines is 
      primarily attributed to the reduced mo-DC migratory capacity. One undefined 
      aspect is the initial binding of mo-DCs to endothelial cells and vascular 
      selectins. In this study, we investigated the role and modulation of the selectin 
      binding determinant sialyl Lewis(x) (sLe(x)) in selectin-dependent mo-DC binding. 
      Our data reveal that sLe(x) is required for maximal binding of mo-DCs to tumor 
      necrosis factor (TNF)-alpha-activated endothelial cells under static conditions, as 
      evidenced by the use of sialidase. Sialidase treatment also abrogated mo-DC cell 
      tethering to immobilized, purified P-, L-, or E-selectin under flow. The 
      requirement of sLe(x)-dependent binding of mo-DC to selectins was further 
      substantiated by using sLe(x) free sugar and anti-sLe(x) antibody, which 
      significantly suppressed mo-DC-selectin binding. P-selectin glycoprotein ligand-1 
      is required for mo-DC binding to both P- and L-selectin, but it is dispensable 
      for E-selectin recognition. Interestingly, the extent of mo-DC tethering was 
      maximal on P-selectin, followed by E- and L- selectin. Accordingly, L-selectin 
      mediated faster mo-DC rolling than E- or P-selectin. Interferon (IFN)-gamma induces a 
      significant increase in mo-DC surface sLe(x) expression, which is probably due to 
      the enhanced synthesis of C2GnT-I. These findings may contribute to improving 
      mo-DC-based vaccination protocols.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Silva, Zelia
AU  - Silva Z
AD  - CEDOC, Departamento de Imunologia, Faculdade de Ciencias Medicas, Universidade 
      Nova de Lisboa, Lisboa, Portugal.
FAU - Tong, ZiQiu
AU  - Tong Z
FAU - Cabral, M Guadalupe
AU  - Cabral MG
FAU - Martins, Catarina
AU  - Martins C
FAU - Castro, Rita
AU  - Castro R
FAU - Reis, Celso
AU  - Reis C
FAU - Trindade, Helder
AU  - Trindade H
FAU - Konstantopoulos, Konstantinos
AU  - Konstantopoulos K
FAU - Videira, Paula A
AU  - Videira PA
LA  - eng
GR  - R01 CA101135/CA/NCI NIH HHS/United States
GR  - R01 CA101135-08/CA/NCI NIH HHS/United States
GR  - U54 CA143868/CA/NCI NIH HHS/United States
GR  - U54 CA143868-02/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110508
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Cancer Vaccines)
RN  - 0 (Oligosaccharides)
RN  - 0 (Selectins)
RN  - 0 (Sialyl Lewis X Antigen)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Cancer Vaccines/immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/drug effects/*immunology
MH  - Humans
MH  - Interferon-gamma/*immunology/pharmacology
MH  - Monocytes/immunology
MH  - Oligosaccharides/*immunology
MH  - Selectins/*immunology
MH  - Sialyl Lewis X Antigen
PMC - PMC3257975
MID - NIHMS300203
EDAT- 2011/05/21 06:00
MHDA- 2011/08/16 06:00
PMCR- 2012/06/10
CRDT- 2011/05/21 06:00
PHST- 2011/04/28 00:00 [received]
PHST- 2011/05/03 00:00 [accepted]
PHST- 2011/05/21 06:00 [entrez]
PHST- 2011/05/21 06:00 [pubmed]
PHST- 2011/08/16 06:00 [medline]
PHST- 2012/06/10 00:00 [pmc-release]
AID - S0006-291X(11)00779-0 [pii]
AID - 10.1016/j.bbrc.2011.05.026 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2011 Jun 10;409(3):459-64. doi: 
      10.1016/j.bbrc.2011.05.026. Epub 2011 May 8.