PMID- 21598134
OWN - NLM
STAT- MEDLINE
DCOM- 20111115
LR  - 20110727
IS  - 1944-0057 (Electronic)
IS  - 1944-0057 (Linking)
VI  - 28
IP  - 8
DP  - 2011 Aug
TI  - Simultaneous analysis of four sulfonamides in chicken muscle tissue by HPLC.
PG  - 1013-20
LID - 10.1080/19440049.2011.577098 [doi]
AB  - The aim of this study was to develop a simple high-performance liquid 
      chromatography (HPLC) with UV detection method for the determination of four 
      sulfonamides in chicken muscle tissue. The sulfonamides were extracted with 
      acetonitrile, acetone and dichloromethane. Separation was carried out on a C18 
      column using as the mobile phase a mixture of 6 per thousand disodium hydrogen phosphate and 
      methanol. The analytes were detected by UV in one run. Calibration curves were 
      linear with very good correlation coefficients for concentration ranging from 30 
      to 150 microg kg(-1). The limits of detection (LOD) for sulfonamides ranged from 6.5 
      to 0.14 microg kg(-1). The recovery for spiked chicken muscle with 50-150 microg kg(-1) 
      was more than 70%. The relative standard deviations (RSDs) of the sulfonamides 
      for six measurements at 50, 100 and 150 microg kg(-1) were less than 15%. These 
      parameters met the European Union criteria for method validation. The results 
      were confirmed by LC-MS/MS using multiple reaction monitoring as the operating 
      mode. Confirmation required the retention times of the analytes to be within 
      +/-2.5% of the retention times of the standards, the presence of the parent ion and 
      two characteristic fragment ions (product ions) per analyte, as well as the 
      relative ion abundance ratios of the fragment ions corresponding to ratios 
      obtained for the standards, within permitted limits. The transition of two common 
      product ions at m/z 155.7 and 107.5 were monitored for all sulfonamides. Each of 
      the analytes, in all tested samples, met the confirmation criteria. Thus, the 
      applicability of the HPLC-UV method for routine analysis of chicken muscle tissue 
      was demonstrated.
FAU - Chitescu, C L
AU  - Chitescu CL
AD  - University Dunarea de Jos Galati, Faculty of Food Science and Engineering, Str. 
      Domneasca 47, 800008 Galati, Romania. chitescucarmenlidia@yahoo.com
FAU - Nicolau, A I
AU  - Nicolau AI
FAU - Csuma, A
AU  - Csuma A
FAU - Moisoiu, C
AU  - Moisoiu C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110523
PL  - England
TA  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess
JT  - Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & 
      risk assessment
JID - 101485040
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Sulfonamides)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Limit of Detection
MH  - Muscles/*chemistry
MH  - Reference Standards
MH  - Sulfonamides/*analysis
MH  - Tandem Mass Spectrometry
EDAT- 2011/05/21 06:00
MHDA- 2011/11/16 06:00
CRDT- 2011/05/21 06:00
PHST- 2011/05/21 06:00 [entrez]
PHST- 2011/05/21 06:00 [pubmed]
PHST- 2011/11/16 06:00 [medline]
AID - 937776143 [pii]
AID - 10.1080/19440049.2011.577098 [doi]
PST - ppublish
SO  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011 
      Aug;28(8):1013-20. doi: 10.1080/19440049.2011.577098. Epub 2011 May 23.