PMID- 21598499
OWN - NLM
STAT- MEDLINE
DCOM- 20150607
LR  - 20181201
IS  - 1000-1182 (Print)
IS  - 1000-1182 (Linking)
VI  - 29
IP  - 2
DP  - 2011 Apr
TI  - [Cloning of the glyceraldehydes 3-phosphate dehydrogenase gene of porphyromonas 
      gingivalis and its expression in E. coli].
PG  - 199-202
AB  - OBJECTIVE: To clone the glyceraldehydes 3-phosphate dehydrogenase (GAPDH) gene of 
      Porphyromonas gingivalis (P. gingivalis) and to induce its fusion expression in 
      E. coli. METHODS: GAPDH was obtained by PCR and was inserted into cloning vector 
      pMD-18-T to construct clone recon. Double enzymes digest the recon pMD18-T-GAPDH 
      and the prokaryotic expression vector pET-32a and then connect to get the 
      expressing recon pET-32a-GAPDH. The recombinant expression plasmid which had been 
      confirmed by enzymes digestion was transformed to E. coli competent cells BL21 
      and induced the expression of GAPDH with isopropyl beta-D-1-thiogalactopyranoside 
      (IPTG) of different density. RESULTS: DNA sequencing showed that the fragment was 
      99.802% the same to the sequence published in NCBI. Under the best density, IPTG 
      could be highly expressed. CONCLUSION: The GAPDH had been successfully cloned and 
      expressed in E. coli which gets ready for the following experiment to study the 
      immunity of GAPDH and the homologues antibody preparation.
FAU - Li, Ang
AU  - Li A
AD  - Research Center for Stomatology, Stomatological Hospital, College of Medicine, 
      Xi'an Jiaotong University, Xi'an 710004, China.
FAU - Xu, Hong-Yan
AU  - Xu HY
FAU - Shi, Jian-Feng
AU  - Shi JF
FAU - Zhu, Chun-Hui
AU  - Zhu CH
FAU - Rao, Guo-Zhou
AU  - Rao GZ
FAU - Gou, Jian-Zhong
AU  - Gou JZ
LA  - chi
PT  - Journal Article
PL  - China
TA  - Hua Xi Kou Qiang Yi Xue Za Zhi
JT  - Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal 
      of stomatology
JID - 9422648
RN  - 0 (Phosphates)
RN  - 367-47-5 (Glyceraldehyde)
RN  - EC 1.- (Oxidoreductases)
SB  - IM
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Cloning, Organism
MH  - *Escherichia coli
MH  - Genetic Vectors
MH  - Glyceraldehyde
MH  - Oxidoreductases
MH  - Phosphates
MH  - Polymerase Chain Reaction
MH  - *Porphyromonas gingivalis
EDAT- 2011/05/24 06:00
MHDA- 2015/06/08 06:00
CRDT- 2011/05/24 06:00
PHST- 2011/05/24 06:00 [entrez]
PHST- 2011/05/24 06:00 [pubmed]
PHST- 2015/06/08 06:00 [medline]
PST - ppublish
SO  - Hua Xi Kou Qiang Yi Xue Za Zhi. 2011 Apr;29(2):199-202.