PMID- 21598515
OWN - NLM
STAT- MEDLINE
DCOM- 20120628
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 2
DP  - 2011 Feb
TI  - [Simultaneous detection of 8 monoamine neurotransmitters in the different 
      sections of rat brains by high performance liquid chromatography with 
      fluorescence detection].
PG  - 146-51
AB  - A method for the simultaneous detection of L-dihydroxyphenylalanine, 
      norepinephrine, epinephrine, dopamine, 3,4-dihydroxyphenylacetic acid, serotonin 
      hydrochloride, 5-hydroxyindole-3-acetic acid and homovanillic acid in the 
      different sections of mouse brains was established by using high performance 
      liquid chromatography (HPLC) with fluorescence detection and isocratic elution. 
      Before analysis, the sample was deproteinized by 0.60 mol/L perchloric acid, 
      followed by adjusting pH value of the sample with 1.20 mol/L K2HPO4, addition of 
      0.1 g/L L-cysteine as antioxidant and 0.50 mmol/L Na2EDTA as complexing agent. 
      The separation column was a Shim-pack C18 column (250 mm x 4.6 mm, 5 microm) and 
      the mobile phase (pH 3.8) was 13% methanol containing 50 mmol/L citric acid, 50 
      mmol/L sodium acetate, 0.5 mmol/L 1-heptanesulfonic acid sodium salt, 5 mmol/L 
      triethylamine and 0.5 mmol/L Na2EDTA. The flow rate was 1.0 mL/min. The injection 
      volume was 10 microL. The emission and excitation wavelengths were 330 nm and 280 
      nm, respectively. Under the optimized separation conditions, the calibration 
      curves showed good linearity within the concentrations of 1.25 - 5000 microg/L (r 
      > 0.9999). The limits of detection were between 0.20 - 5.00 microg/L, the average 
      recoveries were between 94.83% and 99.19%, and the relative standard deviations 
      (RSDs) were between 0.08% and 2.51%. The advantages of the method include easy 
      and prompt operation, high recovery, low detection limit, good separation effect, 
      high accuracy and precision. The method has practical value for detecting 8 
      monoamine neurotransmitters in biological samples.
FAU - Zhao, Yanyan
AU  - Zhao Y
AD  - Experimental Center of Medicine, Hebei University, Baoding 071000, China. 
      zhaoyany606@tom.com
FAU - Liu, Liyan
AU  - Liu L
FAU - Han, Yuanyuan
AU  - Han Y
FAU - Bai, Jie
AU  - Bai J
FAU - Du, Guangling
AU  - Du G
FAU - Gao, Qian
AU  - Gao Q
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Biogenic Monoamines)
RN  - 0 (Neurotransmitter Agents)
RN  - 333DO1RDJY (Serotonin)
RN  - VTD58H1Z2X (Dopamine)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Animals
MH  - Biogenic Monoamines/*analysis
MH  - *Brain Chemistry
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Dopamine/analysis
MH  - Neurotransmitter Agents/*analysis
MH  - Norepinephrine/analysis
MH  - Rats
MH  - Serotonin/analysis
MH  - Spectrometry, Fluorescence/*methods
EDAT- 2011/05/24 06:00
MHDA- 2012/06/29 06:00
CRDT- 2011/05/24 06:00
PHST- 2011/05/24 06:00 [entrez]
PHST- 2011/05/24 06:00 [pubmed]
PHST- 2012/06/29 06:00 [medline]
AID - 10.3724/sp.j.1123.2011.00146 [doi]
PST - ppublish
SO  - Se Pu. 2011 Feb;29(2):146-51. doi: 10.3724/sp.j.1123.2011.00146.