PMID- 21598520
OWN - NLM
STAT- MEDLINE
DCOM- 20120628
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 29
IP  - 2
DP  - 2011 Feb
TI  - [Determination of ethyl glucuronide in human urine by solid phase extraction-gas 
      chromatography-mass spectrometry].
PG  - 172-5
AB  - A solid phase extraction (SPE) and gas chromatography (GC) with mass spectrometry 
      (MS) method for determination of ethyl glucuronide (EtG) in human urine was 
      established. One mL urine sample was deproteinated by 100 microL 3 mol/L 
      hydrochloric acid and cleaned up through a solid phase extraction column. The 
      target analytes were eluted from an NH2-column with 4% ammonia solution and then 
      treated with bis (trimethylsilyl) trifluoroacetamide (BSTFA) + 
      trimethylchlorosilane (TMCS) (99:1) for derivatization. The derivatized samples 
      were analyzed by GC-MS. Data were acquired in the selected ion monitoring (SIM) 
      mode and the quantitation of EtG was done through internal standard method. Good 
      linearity was obtained at the mass concentration range of 0.1 - 3.2 mg/L with a 
      correlation coefficient (r) of 0.9921. The limit of detection (LOD) was 28.4 
      microg/L. The range of recoveries was 92.5% - 108.7%, and the relative standard 
      deviations (RSDs) of intra-day and inter-day were all less than 5%. This method 
      is sensitive, specific, accurate and can be applied to the determination of EtG 
      for medicolegal identification and clinical laboratory.
FAU - Yu, Tianxiao
AU  - Yu T
AD  - Department of Laboratory Medicine, Key Laboratory of Clinical Laboratory 
      Diagnostics of Ministry of Education, Chongqing Medical University, Chongqing 
      400016, China.
FAU - Li, Qing
AU  - Li Q
FAU - Wan, Tao
AU  - Wan T
FAU - Li, Jianbo
AU  - Li J
FAU - Ding, Shijia
AU  - Ding S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Glucuronates)
RN  - 17685-04-0 (ethyl glucuronide)
SB  - IM
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Glucuronates/*urine
MH  - Humans
MH  - Limit of Detection
MH  - Sensitivity and Specificity
MH  - Solid Phase Extraction/*methods
EDAT- 2011/05/24 06:00
MHDA- 2012/06/29 06:00
CRDT- 2011/05/24 06:00
PHST- 2011/05/24 06:00 [entrez]
PHST- 2011/05/24 06:00 [pubmed]
PHST- 2012/06/29 06:00 [medline]
AID - 10.3724/sp.j.1123.2011.00172 [doi]
PST - ppublish
SO  - Se Pu. 2011 Feb;29(2):172-5. doi: 10.3724/sp.j.1123.2011.00172.