PMID- 21600179
OWN - NLM
STAT- MEDLINE
DCOM- 20111205
LR  - 20181201
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 91
IP  - 10
DP  - 2011 Mar 15
TI  - [Growth and migration of umbilical cord mesenchymal stem cells on polycarbonate 
      membrane with different pore sizes].
PG  - 699-702
AB  - OBJECTIVE: To observe the growth and migration of human umbilical cord 
      mesenchymal stem cells (hUCMSCs) on polycarbonate membrane with different pore 
      sizes and explore the criteria of selecting optimal Transwell insert for indirect 
      co-culture to induce the differentiation of hUCMSCs. METHODS: hUCMSCs were 
      isolated in vitro and then expanded in culture medium. After the treatment of 
      mitomycin C, the cells were seeded on porous membranes of 6-well-dish Transwell 
      inserts with different pore sizes of 0.4, 3.0 and 8.0 microm respectively. After 
      culturing for 7 days, the cells were observed and counted on the bottom of each 
      porous membrane. Then the calculation of migration ratio was performed. The 
      growth and migration of hUCMSCs on porous membranes were also examined under 
      scanning electron microscope (SEM). RESULTS: The migration ratios of hUCMSCs on 
      membranes of 0.4, 3.0 and 8.0 microm pore sizes were 0, 1.8% and 8.0% respectively. 
      The migration ratio of cells on 0.4 microm pore size membrane was statistically 
      different from that of the other two pore size groups (P < 0.01). Under SEM, a 
      small portion of cells were growing on the bottoms of membranes and moving 
      through the pores. But there was no cell movement through 0.4 microm pore size 
      membrane. CONCLUSIONS: hUCMSCs can migrate through the polycarbonate membranes of 
      3.0 microm and 8.0 microm pore sizes but not through the 0.4 microm one. Thus both sides of 
      polycarbonate membrane of 0.4 microm pore size may be used for close indirect 
      co-culture to induce the differentiation of hUCMSCs.
FAU - Li, Dong-jie
AU  - Li DJ
AD  - Department of Burns & Plastic Surgery, First Affiliated Hospital of PLA General 
      Hospital, Beijing 100048, China.
FAU - Chai, Jia-ke
AU  - Chai JK
FAU - Han, Yan-fu
AU  - Han YF
FAU - Sun, Tian-jun
AU  - Sun TJ
FAU - Deng, Hu-ping
AU  - Deng HP
FAU - Zhao, Jing-yu
AU  - Zhao JY
FAU - Liu, Ling-ying
AU  - Liu LY
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Polycarboxylate Cement)
RN  - 25766-59-0 (polycarbonate)
SB  - IM
MH  - Cell Culture Techniques
MH  - *Cell Differentiation
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology
MH  - *Polycarboxylate Cement
MH  - Umbilical Cord/cytology
EDAT- 2011/05/24 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/05/24 06:00
PHST- 2011/05/24 06:00 [entrez]
PHST- 2011/05/24 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2011 Mar 15;91(10):699-702.