PMID- 21600247
OWN - NLM
STAT- MEDLINE
DCOM- 20111216
LR  - 20110829
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 160
IP  - 1-2
DP  - 2011 Sep
TI  - Detection of different variants of Ostreid Herpesvirus 1 in the Pacific oyster, 
      Crassostrea gigas between 2008 and 2010.
PG  - 25-31
LID - 10.1016/j.virusres.2011.04.012 [doi]
AB  - Since summer 2008, high mortality rates of young Pacific oysters Crassostrea 
      gigas have been recorded in association with the detection of the Ostreid 
      Herpesvirus 1 (OsHV-1). A new variant called muVar has been recently described, 
      characterized mainly by 12 consecutive deletions followed by one deletion of an 
      adenine in the C region. The purpose of this study is to characterize the 
      genotype (variants or OsHV-1 reference) of 300 positive samples of C. gigas 
      analyzed between July 2008 and July 2010 collected along the French, Jersey, and 
      Irish coasts. Samples were quantified by TaqMan PCR, amplified with conventional 
      PCR, targeting the area of the deletion, and then sequenced. Eighty-seven percent 
      of the samples were characterized and the OsHV-1 muVar was detected in 257 oyster 
      samples. The genotype OsHV-1 reference was never detected during the 25 months of 
      the present survey. Thirty-eight samples could not be determined and the majority 
      of them had a low viral load. A novel genotype containing only 9 consecutive 
      deletions named OsHV-1 muVar Delta9 was found in 5 samples. These observations 
      indicate the emergence of different OsHV-1 variants.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Martenot, Claire
AU  - Martenot C
AD  - Laboratoire Departemental Frank Duncombe, 14053 Caen Cedex 4, France.
FAU - Oden, Elise
AU  - Oden E
FAU - Travaille, Emmanuelle
AU  - Travaille E
FAU - Malas, Jean-Paul
AU  - Malas JP
FAU - Houssin, Maryline
AU  - Houssin M
LA  - eng
PT  - Journal Article
DEP - 20110510
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Atlantic Ocean
MH  - Crassostrea/*virology
MH  - DNA, Viral/chemistry/genetics
MH  - France
MH  - *Genetic Variation
MH  - Genotype
MH  - Herpesviridae/*classification/genetics/*isolation & purification
MH  - Ireland
MH  - New Jersey
MH  - Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Survival Analysis
MH  - Viral Load
EDAT- 2011/05/24 06:00
MHDA- 2011/12/17 06:00
CRDT- 2011/05/24 06:00
PHST- 2011/02/23 00:00 [received]
PHST- 2011/04/15 00:00 [revised]
PHST- 2011/04/16 00:00 [accepted]
PHST- 2011/05/24 06:00 [entrez]
PHST- 2011/05/24 06:00 [pubmed]
PHST- 2011/12/17 06:00 [medline]
AID - S0168-1702(11)00148-1 [pii]
AID - 10.1016/j.virusres.2011.04.012 [doi]
PST - ppublish
SO  - Virus Res. 2011 Sep;160(1-2):25-31. doi: 10.1016/j.virusres.2011.04.012. Epub 
      2011 May 10.