PMID- 21600397
OWN - NLM
STAT- MEDLINE
DCOM- 20110908
LR  - 20220310
IS  - 1873-2259 (Electronic)
IS  - 0168-9452 (Linking)
VI  - 181
IP  - 1
DP  - 2011 Jul
TI  - Effects of osmotic pretreatments on oxidative stress, antioxidant profiles and 
      cryopreservation of olive somatic embryos.
PG  - 47-56
LID - 10.1016/j.plantsci.2011.03.009 [doi]
AB  - A three-day pretreatment of olive somatic embryos (SE) with 0.75 M sucrose, 
      combined with cryoprotection (0.5M DMSO, 1M sucrose, 0.5M glycerol and 0.009 M 
      proline) and controlled rate cooling, supported regrowth (as 34.6% fresh weight 
      gain) and resumption of embryo development after cryopreservation. Pretreatment 
      with mannitol or sorbitol did not support regrowth. Profiles of sugars, proline, 
      antioxidant enzymes, Reactive oxygen species (ROS), secondary oxidation products 
      and ethylene were constructed for the most successful (0.75 M) pretreatment 
      series. Sucrose was the optimal pretreatment for supporting recovery, it also 
      elevated glutathione reductase (GR) activity compared to controls, whereas 
      superoxide dismutase (SOD), catalase and guaiacol peroxidase activities remained 
      relatively unchanged. Superoxide dismutase activity was higher in SE pretreated 
      with sucrose, compared with those pretreated with polyols; H(2)O(2) was enhanced 
      in SE pretreated with sorbitol and sucrose compared to mannitol. The overall 
      trend for ethylene and OH production revealed their levels were highest in SE 
      pretreated with polyols albeit, for individual treatments this was not always the 
      case. Generally, pretreatments did not significantly change embryo secondary 
      oxidation profiles of ThioBarbituric Acid Reactive Substances (TBARS) and 
      Schiff's bases. In combination these studies suggest oxidative processes may 
      influence regrowth of cryopreserved olive SE and that optimal pretreatments 
      could, in part, increase tolerance by an overall enhancement of endogenous 
      antioxidants (particularly GR), proline and sugars.
CI  - Copyright (c) 2011 Elsevier Ireland Ltd. All rights reserved.
FAU - Lynch, Paul T
AU  - Lynch PT
AD  - Biological Sciences Research Group, University of Derby, Kedleston Road, Derby 
      DE22 1GB, UK. P.T.Lynch@derby.ac.uk
FAU - Siddika, Ayesha
AU  - Siddika A
FAU - Johnston, Jason W
AU  - Johnston JW
FAU - Trigwell, Susan M
AU  - Trigwell SM
FAU - Mehra, Aradhana
AU  - Mehra A
FAU - Benelli, Carla
AU  - Benelli C
FAU - Lambardi, Maurizio
AU  - Lambardi M
FAU - Benson, Erica E
AU  - Benson EE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110401
PL  - Ireland
TA  - Plant Sci
JT  - Plant science : an international journal of experimental plant biology
JID - 9882015
RN  - 0 (Antioxidants)
RN  - 0 (Ethylenes)
RN  - 0 (Polymers)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Schiff Bases)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 0 (polyol)
RN  - 3OWL53L36A (Mannitol)
RN  - 506T60A25R (Sorbitol)
RN  - 57-50-1 (Sucrose)
RN  - 91GW059KN7 (ethylene)
RN  - 9DLQ4CIU6V (Proline)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.- (guaiacol peroxidase)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.8.1.7 (Glutathione Reductase)
SB  - IM
MH  - Antioxidants/*metabolism
MH  - Catalase/metabolism
MH  - *Cryopreservation
MH  - Ethylenes/metabolism
MH  - Glutathione Reductase/metabolism
MH  - Hydrogen Peroxide/metabolism
MH  - Mannitol/metabolism
MH  - Olea/enzymology/*growth & development/metabolism
MH  - *Osmosis
MH  - *Oxidative Stress
MH  - Peroxidase/metabolism
MH  - Plant Somatic Embryogenesis Techniques
MH  - Polymers/metabolism
MH  - Proline/analysis
MH  - Reactive Oxygen Species/metabolism
MH  - Schiff Bases/analysis
MH  - Sorbitol/metabolism
MH  - Sucrose/metabolism
MH  - Superoxide Dismutase/metabolism
MH  - Thiobarbituric Acid Reactive Substances/analysis
EDAT- 2011/05/24 06:00
MHDA- 2011/09/09 06:00
CRDT- 2011/05/24 06:00
PHST- 2010/07/15 00:00 [received]
PHST- 2011/03/12 00:00 [revised]
PHST- 2011/03/15 00:00 [accepted]
PHST- 2011/05/24 06:00 [entrez]
PHST- 2011/05/24 06:00 [pubmed]
PHST- 2011/09/09 06:00 [medline]
AID - S0168-9452(11)00086-0 [pii]
AID - 10.1016/j.plantsci.2011.03.009 [doi]
PST - ppublish
SO  - Plant Sci. 2011 Jul;181(1):47-56. doi: 10.1016/j.plantsci.2011.03.009. Epub 2011 
      Apr 1.