PMID- 21602125
OWN - NLM
STAT- MEDLINE
DCOM- 20130304
LR  - 20181201
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 31
IP  - 5
DP  - 2011 May
TI  - [Effect of ecdysterone on the proliferation of human umbilical cord mesenchymal 
      stem cells in vitro].
PG  - 787-90
AB  - OBJECTIVE: To investigate the effect of ecdysterone on the proliferation of human 
      umbilical cord mesenchymal stem cells (hUCMSCs) in vitro. METHODS: hUCMSCs 
      isolated by enzyme digestion from human umbilical cord tissues were cultured and 
      identified for the surface antigens using fluorescence-activated cell sorting 
      (FACS). The cells were treated with ecdysterone at the concentrations of 0, 25, 
      50, 100, 150, and 200 microg/ml, and the changes in the cell proliferation were 
      detected using MTT assay. RESULTS: The third-passage hUCMSCs were positive for 
      CD29 and CD105 and negative for CD34 and CD45 as shown by flow cytometry. 
      Treatment with ecdysterone resulted in significantly increased cell proliferation 
      as compared to the control cells (P<0.05), but no significant differences were 
      found in cells treated with 100, 150, and 200 microg/ml ecdysterone (P>0.05). The 
      growth curves of the cells also demonstrated the definite effect of ecdysterone 
      in promoting the proliferation of hUCMSCs. CONCLUSION: Ecdysterone can promote 
      the proliferation of hUCMSCs in vitro with the optimal concentration of 100 
      microg/ml, suggesting its potential value in the enrichment of mesenchymal stem 
      cells.
FAU - Zhang, Yuan-zhong
AU  - Zhang YZ
AD  - Department of Thoracic and Cardiovascular Surgery, Southern Medical University, 
      Guangzhou, China. zyz518836@163.com
FAU - Fu, Xiao-bing
AU  - Fu XB
FAU - Huang, Sha
AU  - Huang S
FAU - Ma, Kui
AU  - Ma K
FAU - Zhang, Zi-liang
AU  - Zhang ZL
FAU - Feng, Chang-jiang
AU  - Feng CJ
FAU - Wu, Xu
AU  - Wu X
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 5289-74-7 (Ecdysterone)
SB  - IM
MH  - Cell Proliferation/*drug effects
MH  - Cells, Cultured
MH  - Ecdysterone/*pharmacology
MH  - Flow Cytometry
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/drug effects
MH  - Umbilical Cord/*cytology/drug effects
EDAT- 2011/05/24 06:00
MHDA- 2013/03/05 06:00
CRDT- 2011/05/24 06:00
PHST- 2011/05/24 06:00 [entrez]
PHST- 2011/05/24 06:00 [pubmed]
PHST- 2013/03/05 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2011 May;31(5):787-90.