PMID- 21602217
OWN - NLM
STAT- MEDLINE
DCOM- 20111122
LR  - 20200826
IS  - 1465-2080 (Electronic)
IS  - 1350-0872 (Linking)
VI  - 157
IP  - Pt 8
DP  - 2011 Aug
TI  - Identification of a novel subgroup of uncultured gammaproteobacterial 
      glycogen-accumulating organisms in enhanced biological phosphorus removal sludge.
PG  - 2287-2296
LID - 10.1099/mic.0.047779-0 [doi]
AB  - The presence of glycogen-accumulating organisms (GAO) has been hypothesized to be 
      a cause of deterioration in enhanced biological phosphorus removal (EBPR) 
      processes due to their abilities to out-compete polyphosphate-accumulating 
      organisms (PAO). Based on 16S rRNA gene sequences, new members of uncultured 
      gammaproteobacterial GAO (GB) were identified from sludge samples of a lab-scale 
      sequencing batch reactor used for EBPR. The new GB formed a phylogenetic lineage 
      (GB8) clearly distinct from the previously reported seven GB subgroups. Because 
      the new GB8 members were not targeted by the known fluorescence in situ 
      hybridization (FISH) oligonucleotide probes, a GB8-specific FISH probe (GB429) 
      and a new FISH probe (GB742) targeting all eight GB subgroups were designed, and 
      the phenotypic properties of the new GB8 members were investigated. FISH and 
      microautoradiography approaches showed that GB429-targeted cells (GB8) were large 
      coccobacilli (2-4 microm in size) with the ability to take up acetate under anaerobic 
      conditions, but unable to accumulate polyphosphate under the subsequent aerobic 
      conditions, consistent with in situ phenotypes of GB. FISH analyses on several 
      sludge samples showed that members of GB8 were commonly detected as the majority 
      of GB in lab- and full-scale EBPR processes. In conclusion, this study showed 
      that members of GB8 could be a subgroup of GB with an important role in EBPR 
      deterioration. Designs of FISH probes which hybridize with broader GB subgroups 
      at different hierarchical levels will contribute to studies of the distributions 
      and ecophysiologies of GB in lab- or full-scale EBPR plants.
FAU - Kim, Jeong Myeong
AU  - Kim JM
AD  - School of Biological Sciences, Chung-Ang University, HeukSeok-Dong, Dongjak-Gu, 
      Seoul, 156-756, Republic of Korea.
FAU - Lee, Hyo Jung
AU  - Lee HJ
AD  - School of Biological Sciences, Chung-Ang University, HeukSeok-Dong, Dongjak-Gu, 
      Seoul, 156-756, Republic of Korea.
FAU - Lee, Dae Sung
AU  - Lee DS
AD  - Department of Environmental Engineering, Kyungpook National University, 
      Sankyuk-dong, Buk-gu, Daegu 702-701, Republic of Korea.
FAU - Lee, Kangseok
AU  - Lee K
AD  - School of Biological Sciences, Chung-Ang University, HeukSeok-Dong, Dongjak-Gu, 
      Seoul, 156-756, Republic of Korea.
FAU - Jeon, Che Ok
AU  - Jeon CO
AD  - School of Biological Sciences, Chung-Ang University, HeukSeok-Dong, Dongjak-Gu, 
      Seoul, 156-756, Republic of Korea.
LA  - eng
SI  - GENBANK/HQ341387
SI  - GENBANK/HQ341388
SI  - GENBANK/HQ341389
SI  - GENBANK/HQ341390
SI  - GENBANK/HQ341391
SI  - GENBANK/HQ341392
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110520
PL  - England
TA  - Microbiology (Reading)
JT  - Microbiology (Reading, England)
JID - 9430468
RN  - 0 (Acetates)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 27YLU75U4W (Phosphorus)
RN  - 9005-79-2 (Glycogen)
SB  - IM
MH  - Acetates/metabolism
MH  - Anaerobiosis
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Gammaproteobacteria/*classification/genetics/isolation & purification/*metabolism
MH  - Glycogen/*metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Phosphorus/*metabolism
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sewage/*microbiology
EDAT- 2011/05/24 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/05/24 06:00
PHST- 2011/05/24 06:00 [entrez]
PHST- 2011/05/24 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - 10.1099/mic.0.047779-0 [doi]
PST - ppublish
SO  - Microbiology (Reading). 2011 Aug;157(Pt 8):2287-2296. doi: 
      10.1099/mic.0.047779-0. Epub 2011 May 20.