PMID- 21604389
OWN - NLM
STAT- MEDLINE
DCOM- 20110701
LR  - 20200716
IS  - 0372-9311 (Print)
IS  - 0372-9311 (Linking)
IP  - 2
DP  - 2011 Mar-Apr
TI  - [Evaluation of toxin producing abilities of non-O1/non-O139 serogroup Vibrio 
      cholerae isolated from humans].
PG  - 8-12
AB  - AIM: Determination of non-O1/non-O139 Vibrio cholerae toxin (CT) gene expression 
      by using EIA, and biological effect of non-O1/non-O139 V. cholerae supernatant on 
      cell cultures evaluation. MATERIALS AND METHODS: 39 V. cholerae strains from 
      various serological groups were studied. Hemolytic activity of strains was 
      determined by using Greig test, and cholera toxin production--in GM1-EIA and in 
      continuous cell lines by registering cytotonic, cytotoxic and proteolitic effect. 
      RESULTS: GM1-EIA method does not detect CT production in 29 museum strains of 
      non-O1/non-O139 V. cholerae in vitro. CT was detected only in 1 non-O1/non-O139 
      V. cholerae strain supernatant with OD = 0.577 that is substantially lower than 
      in O1 V. cholerae strains (OD = 2.176). In cell cultures non-O1/non-O139 V. 
      cholerae supernatants diluted to 1:100 caused elongation only in single cells. 
      CONCLUSION: Cytological model is a more sensitive technique to evaluate toxin 
      producing abilities of non-O1/non-O139 V. cholerae strains and is appropriate for 
      use.
FAU - Telesmanich, N R
AU  - Telesmanich NR
FAU - Akulova, M V
AU  - Akulova MV
FAU - Lomov, Iu M
AU  - Lomov IuM
FAU - Alekseeva, L P
AU  - Alekseeva LP
FAU - Kruglikova, V D
AU  - Kruglikova VD
FAU - Agafonova, V V
AU  - Agafonova VV
FAU - Tatarenko, O A
AU  - Tatarenko OA
FAU - Evdokimova, V V
AU  - Evdokimova VV
LA  - rus
PT  - Journal Article
PL  - Russia (Federation)
TA  - Zh Mikrobiol Epidemiol Immunobiol
JT  - Zhurnal mikrobiologii, epidemiologii i immunobiologii
JID - 0415217
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (ganglioside receptor)
RN  - 9012-63-9 (Cholera Toxin)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Cell Culture Techniques
MH  - Cell Shape
MH  - Cholera/immunology/pathology
MH  - Cholera Toxin/*biosynthesis/immunology/metabolism
MH  - Cricetinae
MH  - Cricetulus
MH  - Erythrocytes/metabolism
MH  - Fluorescent Antibody Technique, Direct
MH  - Hemolysis
MH  - Humans
MH  - Receptors, Cell Surface/immunology/metabolism
MH  - Structure-Activity Relationship
MH  - Vibrio cholerae O1/chemistry/*immunology
MH  - Vibrio cholerae non-O1/chemistry/*immunology
EDAT- 2011/05/24 06:00
MHDA- 2011/07/02 06:00
CRDT- 2011/05/24 06:00
PHST- 2011/05/24 06:00 [entrez]
PHST- 2011/05/24 06:00 [pubmed]
PHST- 2011/07/02 06:00 [medline]
PST - ppublish
SO  - Zh Mikrobiol Epidemiol Immunobiol. 2011 Mar-Apr;(2):8-12.