PMID- 21604966
OWN - NLM
STAT- MEDLINE
DCOM- 20111014
LR  - 20180924
IS  - 1460-2202 (Electronic)
IS  - 0271-3683 (Linking)
VI  - 36
IP  - 7
DP  - 2011 Jul
TI  - Ameliorative effect of thymoquinone on ovalbumin-induced allergic conjunctivitis 
      in Balb/c mice.
PG  - 591-8
LID - 10.3109/02713683.2011.573898 [doi]
AB  - OBJECTIVE: Thymoquinone (TQ) is an active and potent compound in the oil of 
      Nigella sativa, which has anti-inflammatory properties. The aim of this study was 
      to evaluate the effects of TQ on ovalbumin (OVA)-induced allergic conjunctivitis 
      (AC) in Balb/c. METHODS: The mice were divided into seven experimental groups; 
      PBS Con, OVA Con, Conj, 0.05%TQ, 0.1%TQ, 0.5%TQ and Dex group. The mice were 
      immunized and exposed to OVA, and eyes were treated with TQ and dexamethasone. 
      Ocular symptoms were observed after last exposure to OVA. Eosinophils count in 
      blood and Ophthalmic lavage fluid (OLF), recruitment of inflammatory cells in 
      conjunctiva, serum IgE and OVA-specific IgE were evaluated by Giemsa and HE 
      staining, and Enzyme-Linked Immunosorbent Assay (ELISA) respectively. High 
      Performance Liquid Chromatography (HPLC) was used to determine the histamine 
      level in OLF. The mRNA expression and protein level of cytokines were examined by 
      real time RT-PCR and ELISA respectively. RESULTS: Ocular symptoms of AC and other 
      characteristics of allergic inflammation including IgE and OVA-specific 
      immunoglobulin E (IgE) level, recruitment of eosinophils, histamine level, mRNA 
      expressions and protein level of cytokines were remarkably increased in 
      OVA-exposed mice compared with the control groups. Administration of TQ 
      suppressed the ocular symptoms, inflammatory cell infiltration in conjunctiva, 
      blood and OLF, increased level of serum IgE and OVA-specific IgE, and OLF 
      histamine level in OVA-exposed mice. Furthermore, TQ abrogated the mRNA 
      expression and serum level of interleukin including 1L-4, IL-5, IL-13 and 
      transforming growth factor beta (TGF-beta) in mice immunized and exposed to OVA. 
      CONCLUSION: Administration of TQ significantly reduced the ocular symptoms in AC 
      by attenuating the recruitment of eosinophils, level of IgE, histamine and 
      cytokines. Our findings suggest that TQ might be a useful intimation for the 
      treatment and future research for AC.
FAU - Hayat, Khizar
AU  - Hayat K
AD  - Department of Ophthalmology, Second Affiliated Hospital, College of Medicine 
      Xi'an Jiaotong University, Shaanxi, People's Republic of China.
FAU - Asim, M B Raza
AU  - Asim MB
FAU - Nawaz, Muhammad
AU  - Nawaz M
FAU - Li, Miao
AU  - Li M
FAU - Zhang, Lin
AU  - Zhang L
FAU - Sun, Naixue
AU  - Sun N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110523
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Benzoquinones)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 820484N8I3 (Histamine)
RN  - 9006-59-1 (Ovalbumin)
RN  - O60IE26NUF (thymoquinone)
SB  - IM
MH  - Animals
MH  - Benzoquinones/administration & dosage/*pharmacology
MH  - Chromatography, High Pressure Liquid
MH  - Conjunctivitis, Allergic/chemically induced/immunology/*prevention & control
MH  - Cytokines/genetics/metabolism
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Eosinophils/immunology
MH  - Histamine/metabolism
MH  - Immunoglobulin E/immunology
MH  - Leukocyte Count
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2011/05/25 06:00
MHDA- 2011/10/15 06:00
CRDT- 2011/05/25 06:00
PHST- 2011/05/25 06:00 [entrez]
PHST- 2011/05/25 06:00 [pubmed]
PHST- 2011/10/15 06:00 [medline]
AID - 10.3109/02713683.2011.573898 [doi]
PST - ppublish
SO  - Curr Eye Res. 2011 Jul;36(7):591-8. doi: 10.3109/02713683.2011.573898. Epub 2011 
      May 23.