PMID- 21606208
OWN - NLM
STAT- MEDLINE
DCOM- 20110830
LR  - 20220129
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 193
IP  - 5
DP  - 2011 May 30
TI  - Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle 
      neutralization before exocytosis.
PG  - 831-9
LID - 10.1083/jcb.201009119 [doi]
AB  - WASP and SCAR homologue (WASH) is a recently identified and evolutionarily 
      conserved regulator of actin polymerization. In this paper, we show that WASH 
      coats mature Dictyostelium discoideum lysosomes and is essential for exocytosis 
      of indigestible material. A related process, the expulsion of the lethal 
      endosomal pathogen Cryptococcus neoformans from mammalian macrophages, also uses 
      WASH-coated vesicles, and cells expressing dominant negative WASH mutants 
      inefficiently expel C. neoformans. D. discoideum WASH causes filamentous actin 
      (F-actin) patches to form on lysosomes, leading to the removal of vacuolar 
      adenosine triphosphatase (V-ATPase) and the neutralization of lysosomes to form 
      postlysosomes. Without WASH, no patches or coats are formed, neutral 
      postlysosomes are not seen, and indigestible material such as dextran is not 
      exocytosed. Similar results occur when actin polymerization is blocked with 
      latrunculin. V-ATPases are known to bind avidly to F-actin. Our data imply a new 
      mechanism, actin-mediated sorting, in which WASH and the Arp2/3 complex 
      polymerize actin on vesicles to drive the separation and recycling of proteins 
      such as the V-ATPase.
FAU - Carnell, Michael
AU  - Carnell M
AD  - Cancer Research UK Beatson Institute for Cancer Research, Bearsden, Glasgow G61 
      1BD, Scotland, UK.
FAU - Zech, Tobias
AU  - Zech T
FAU - Calaminus, Simon D
AU  - Calaminus SD
FAU - Ura, Seiji
AU  - Ura S
FAU - Hagedorn, Monica
AU  - Hagedorn M
FAU - Johnston, Simon A
AU  - Johnston SA
FAU - May, Robin C
AU  - May RC
FAU - Soldati, Thierry
AU  - Soldati T
FAU - Machesky, Laura M
AU  - Machesky LM
FAU - Insall, Robert H
AU  - Insall RH
LA  - eng
GR  - G0601171/MRC_/Medical Research Council/United Kingdom
GR  - G117/569/MRC_/Medical Research Council/United Kingdom
GR  - 088148MF/WT_/Wellcome Trust/United Kingdom
GR  - CRUK_/Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110523
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Actins)
RN  - 0 (Microfilament Proteins)
RN  - EC 3.6.1.- (Vacuolar Proton-Translocating ATPases)
SB  - IM
MH  - Actins/*chemistry/*metabolism
MH  - Cryptococcus neoformans/metabolism/pathogenicity
MH  - Dictyostelium/cytology/metabolism
MH  - *Exocytosis
MH  - Lysosomes/metabolism
MH  - Microfilament Proteins/*metabolism
MH  - Vacuolar Proton-Translocating ATPases/*metabolism
PMC - PMC3105540
EDAT- 2011/05/25 06:00
MHDA- 2011/08/31 06:00
PMCR- 2011/11/30
CRDT- 2011/05/25 06:00
PHST- 2011/05/25 06:00 [entrez]
PHST- 2011/05/25 06:00 [pubmed]
PHST- 2011/08/31 06:00 [medline]
PHST- 2011/11/30 00:00 [pmc-release]
AID - jcb.201009119 [pii]
AID - 201009119 [pii]
AID - 10.1083/jcb.201009119 [doi]
PST - ppublish
SO  - J Cell Biol. 2011 May 30;193(5):831-9. doi: 10.1083/jcb.201009119. Epub 2011 May 
      23.