PMID- 21607023
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20220317
IS  - 1177-5483 (Electronic)
IS  - 1177-5467 (Print)
IS  - 1177-5467 (Linking)
VI  - 5
DP  - 2011
TI  - Tumor necrosis factor-alpha (TNF-alpha) levels in aqueous humor of primary open angle 
      glaucoma.
PG  - 553-6
LID - 10.2147/OPTH.S19453 [doi]
AB  - PURPOSE: Tumor necrosis factor alpha (TNF-alpha), a macrophage/monocyte derived 
      pluripotent cytokine is associated with tissue ischemia, neuronal damage and 
      remodeling. The physiological level of TNF-alpha in aqueous humor of normal and 
      glaucomatous eyes is unknown. In this study, we evaluated the TNF-alpha levels in 
      aqueous in patients with primary open angle glaucoma (POAG) and compared them to 
      controls. METHODS: 50-100 muL of undiluted aqueous humor samples were obtained 
      from eyes of 32 POAG patients who underwent cataract extraction, trabeculectomy 
      or aqueous shunt implantation. Controls were obtained from 32 normal subjects who 
      underwent routine cataract surgery. TNF-alpha levels were quantified using singleplex 
      bead immunoassay analysis. RESULTS: The average TNF-alpha level in POAG samples was 
      2.72 +/- 1.5 pg/mL (mean +/- SD). The average TNF-alpha level in normal samples was 1.59 
      +/- 0.46 pg/mL (mean +/- SD). Significant increase of TNF-alpha levels in POAG samples 
      was noted in comparison to normal samples (P < 0.001). CONCLUSION: TNF-alpha levels 
      are elevated in aqueous in patients with POAG compared to normal subjects based 
      on highly sensitive Luminex((R)) bead immunoassay and may be a reliable biomarker 
      in the progression of glaucoma.
FAU - Balaiya, Sankarathi
AU  - Balaiya S
AD  - University of Florida College of Medicine, Department of Ophthalmology 
      Jacksonville, FL, USA.
FAU - Edwards, Jayson
AU  - Edwards J
FAU - Tillis, Tina
AU  - Tillis T
FAU - Khetpal, Vijay
AU  - Khetpal V
FAU - Chalam, Kakarla V
AU  - Chalam KV
LA  - eng
PT  - Journal Article
DEP - 20110429
PL  - New Zealand
TA  - Clin Ophthalmol
JT  - Clinical ophthalmology (Auckland, N.Z.)
JID - 101321512
PMC - PMC3096616
OTO - NOTNLM
OT  - POAG
OT  - TNF-alpha
OT  - aqueous humor
OT  - cytokine
EDAT- 2011/05/25 06:00
MHDA- 2011/05/25 06:01
PMCR- 2011/04/29
CRDT- 2011/05/25 06:00
PHST- 2011/04/28 00:00 [received]
PHST- 2011/05/25 06:00 [entrez]
PHST- 2011/05/25 06:00 [pubmed]
PHST- 2011/05/25 06:01 [medline]
PHST- 2011/04/29 00:00 [pmc-release]
AID - opth-5-553 [pii]
AID - 10.2147/OPTH.S19453 [doi]
PST - ppublish
SO  - Clin Ophthalmol. 2011;5:553-6. doi: 10.2147/OPTH.S19453. Epub 2011 Apr 29.