PMID- 21608101
OWN - NLM
STAT- MEDLINE
DCOM- 20120413
LR  - 20131121
IS  - 1522-7243 (Electronic)
IS  - 1522-7235 (Linking)
VI  - 26
IP  - 6
DP  - 2011 Nov-Dec
TI  - Gold nanoparticles-based fluorescence enhancement of the terbium-levofloxacin 
      system and its application in pharmaceutical preparations.
PG  - 768-73
LID - 10.1002/bio.1311 [doi]
AB  - A sensitive fluorescence (FL) technique is proposed for the determination of 
      levofloxacin (LVX). The method is based on the fact that the weak FL signal of 
      the Tb(III)-LVX system is strongly enhanced in the presence of gold 
      nanoparticles. Gold nanoparticles were prepared by the citrate reduction of 
      HAuCl(4) and characterized by transmission electron microscopy (TEM). 
      Levofloxacin and Tb(III) ion form a fluorescence complex in aqueous solution, and 
      its maximum emission wavelength was found at 545 nm. Optimal conditions for the 
      formation of the levofloxacin-Tb(III) complexes were studied. Levofloxacin was 
      detected by measuring the FL intensity, which increases linearly with the 
      concentration of LVX in the range 6.2 x 10(-10)-2.6 x 10(-8) mol/L. Recovery of 
      the target analytes was > 96% with good quality parameters: linearity (r(2) > 
      0.996), limit of detection (LOD) and limit of quantification (LOQ) values 2.1 x 
      10(-10) mol/L and 7.2 x 10(-10) mol/L, and run-to-run and day-to-day precisions 
      with relative standard deviations (RSDs) around 3%. Thus, the proposed method can 
      be successfully applied to the routine determination of levofloxacin in 
      pharmaceutical preparations.
CI  - Copyright (c) 2011 John Wiley & Sons, Ltd.
FAU - Lee, Sang Hak
AU  - Lee SH
AD  - Department of Chemistry, Kyungpook National University, Taegu, 702-701, Korea.
FAU - Wabaidur, Saikh Mohammad
AU  - Wabaidur SM
FAU - Alothman, Zeid Abdullah
AU  - Alothman ZA
FAU - Alam, Seikh Mafiz
AU  - Alam SM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110524
PL  - England
TA  - Luminescence
JT  - Luminescence : the journal of biological and chemical luminescence
JID - 100889025
RN  - 0 (Anti-Bacterial Agents)
RN  - 06SSF7P179 (Terbium)
RN  - 6GNT3Y5LMF (Levofloxacin)
RN  - 7440-57-5 (Gold)
RN  - A4P49JAZ9H (Ofloxacin)
SB  - IM
MH  - Anti-Bacterial Agents/*chemistry
MH  - Fluorescence
MH  - Gold/*chemistry
MH  - *Levofloxacin
MH  - Limit of Detection
MH  - *Metal Nanoparticles
MH  - Microscopy, Electron, Transmission
MH  - Ofloxacin/*chemistry
MH  - Terbium/*chemistry
EDAT- 2011/05/25 06:00
MHDA- 2012/04/14 06:00
CRDT- 2011/05/25 06:00
PHST- 2011/01/05 00:00 [received]
PHST- 2011/02/28 00:00 [revised]
PHST- 2011/04/06 00:00 [accepted]
PHST- 2011/05/25 06:00 [entrez]
PHST- 2011/05/25 06:00 [pubmed]
PHST- 2012/04/14 06:00 [medline]
AID - 10.1002/bio.1311 [doi]
PST - ppublish
SO  - Luminescence. 2011 Nov-Dec;26(6):768-73. doi: 10.1002/bio.1311. Epub 2011 May 24.