PMID- 21609454
OWN - NLM
STAT- MEDLINE
DCOM- 20110926
LR  - 20211020
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 12
DP  - 2011 May 24
TI  - Transcriptomic assessment of resistance to effects of an aryl hydrocarbon 
      receptor (AHR) agonist in embryos of Atlantic killifish (Fundulus heteroclitus) 
      from a marine Superfund site.
PG  - 263
LID - 10.1186/1471-2164-12-263 [doi]
AB  - BACKGROUND: Populations of Atlantic killifish (Fundulus heteroclitus) have 
      evolved resistance to the embryotoxic effects of polychlorinated biphenyls (PCBs) 
      and other halogenated and nonhalogenated aromatic hydrocarbons that act through 
      an aryl hydrocarbon receptor (AHR)-dependent signaling pathway. The resistance is 
      accompanied by reduced sensitivity to induction of cytochrome P450 1A (CYP1A), a 
      widely used biomarker of aromatic hydrocarbon exposure and effect, but whether 
      the reduced sensitivity is specific to CYP1A or reflects a genome-wide reduction 
      in responsiveness to all AHR-mediated changes in gene expression is unknown. We 
      compared gene expression profiles and the response to 
      3,3',4,4',5-pentachlorobiphenyl (PCB-126) exposure in embryos (5 and 10 dpf) and 
      larvae (15 dpf) from F. heteroclitus populations inhabiting the New Bedford 
      Harbor, Massachusetts (NBH) Superfund site (PCB-resistant) and a reference site, 
      Scorton Creek, Massachusetts (SC; PCB-sensitive). RESULTS: Analysis using a 
      7,000-gene cDNA array revealed striking differences in responsiveness to PCB-126 
      between the populations; the differences occur at all three stages examined. 
      There was a sizeable set of PCB-responsive genes in the sensitive SC population, 
      a much smaller set of PCB-responsive genes in NBH fish, and few similarities in 
      PCB-responsive genes between the two populations. Most of the array results were 
      confirmed, and additional PCB-regulated genes identified, by RNA-Seq (deep 
      pyrosequencing). CONCLUSIONS: The results suggest that NBH fish possess a gene 
      regulatory defect that is not specific to one target gene such as CYP1A but 
      rather lies in a regulatory pathway that controls the transcriptional response of 
      multiple genes to PCB exposure. The results are consistent with genome-wide 
      disruption of AHR-dependent signaling in NBH fish.
FAU - Oleksiak, Marjorie F
AU  - Oleksiak MF
AD  - Rosenstiel School of Marine and Atmospheric Sciences, University of Miami, 4600 
      Rickenbacker Causeway, Miami, FL 33149 USA.
FAU - Karchner, Sibel I
AU  - Karchner SI
FAU - Jenny, Matthew J
AU  - Jenny MJ
FAU - Franks, Diana G
AU  - Franks DG
FAU - Welch, David B Mark
AU  - Welch DB
FAU - Hahn, Mark E
AU  - Hahn ME
LA  - eng
GR  - P42 ES007381/ES/NIEHS NIH HHS/United States
GR  - P42ES007381/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110524
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - EC 1.14.14.1 (Aryl Hydrocarbon Hydroxylases)
RN  - TSH69IA9XF (3,4,5,3',4'-pentachlorobiphenyl)
SB  - IM
MH  - Animals
MH  - Aryl Hydrocarbon Hydroxylases/biosynthesis
MH  - Drug Resistance/*genetics
MH  - Embryo, Nonmammalian/*drug effects/*metabolism
MH  - Enzyme Induction/drug effects/genetics
MH  - Fundulidae/*embryology/*genetics
MH  - *Gene Expression Profiling
MH  - Genome/genetics
MH  - High-Throughput Nucleotide Sequencing
MH  - Larva/drug effects/genetics
MH  - Oceans and Seas
MH  - Oligonucleotide Array Sequence Analysis
MH  - Polychlorinated Biphenyls/toxicity
MH  - Receptors, Aryl Hydrocarbon/*agonists
MH  - Sequence Analysis, RNA
MH  - Time Factors
PMC - PMC3213123
EDAT- 2011/05/26 06:00
MHDA- 2011/09/29 06:00
PMCR- 2011/05/24
CRDT- 2011/05/26 06:00
PHST- 2011/01/26 00:00 [received]
PHST- 2011/05/24 00:00 [accepted]
PHST- 2011/05/26 06:00 [entrez]
PHST- 2011/05/26 06:00 [pubmed]
PHST- 2011/09/29 06:00 [medline]
PHST- 2011/05/24 00:00 [pmc-release]
AID - 1471-2164-12-263 [pii]
AID - 10.1186/1471-2164-12-263 [doi]
PST - epublish
SO  - BMC Genomics. 2011 May 24;12:263. doi: 10.1186/1471-2164-12-263.