PMID- 2161152
OWN - NLM
STAT- MEDLINE
DCOM- 19900705
LR  - 20190714
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 176
IP  - 2
DP  - 1990 Jun
TI  - Analysis of a glycoprotein of human herpesvirus 6 (HHV-6) using monoclonal 
      antibodies.
PG  - 625-8
AB  - The virus-specific polypeptides of human herpesvirus 6 (HHV-6) were studied. Six 
      hybridoma clones secreting monoclonal antibodies were established. Localized 
      cytoplasmic antigen and surface antigens were found in and on the infected cells, 
      respectively, by using the immunofluorescent test with those antibodies. In the 
      neutralization test, two clones (OHV3 and OHV9) neutralized HHV-6, but the other 
      five monoclonal antibodies did not. When infected cells were labeled with 
      [35S]methionine and the antigens were immunoprecipitated by OHV3 and OHV9 
      followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, 
      two polypeptides with molecular weights of 98,000 (98K) and 92K were detected. 
      Furthermore, it was found that these polypeptides were glycosylated, because they 
      were labeled with [14C]mannose. Pulse-chase studies revealed that precursor 
      molecules were cotranslationaly glycosylated to produce 98K glycoprotein and they 
      were replaced by 92K glycoprotein. Endo-beta-N-acetylglucosaminidases H and F 
      reduced those glycoproteins to putative precursor molecules of 75 kDa. This 
      indicates that N-linked high-mannose sugars associate to 98K and 92K 
      glycoproteins.
FAU - Okuno, T
AU  - Okuno T
AD  - Department of Virology, Research Institute for Microbial Diseases, Osaka 
      University, Japan.
FAU - Sao, H
AU  - Sao H
FAU - Asada, H
AU  - Asada H
FAU - Shiraki, K
AU  - Shiraki K
FAU - Takahashi, M
AU  - Takahashi M
FAU - Yamanishi, K
AU  - Yamanishi K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Glycoproteins)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Cells, Cultured
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fetal Blood/cytology
MH  - Fluorescent Antibody Technique
MH  - Glycoproteins/*analysis/immunology
MH  - Herpesvirus 6, Human/*analysis/immunology
MH  - Humans
MH  - Hybridomas
MH  - Lymphocytes/microbiology
MH  - Mice
MH  - Neutralization Tests
MH  - Precipitin Tests
MH  - Viral Proteins/*analysis/immunology
EDAT- 1990/06/01 00:00
MHDA- 1990/06/01 00:01
CRDT- 1990/06/01 00:00
PHST- 1990/06/01 00:00 [pubmed]
PHST- 1990/06/01 00:01 [medline]
PHST- 1990/06/01 00:00 [entrez]
AID - 10.1016/0042-6822(90)90033-n [doi]
PST - ppublish
SO  - Virology. 1990 Jun;176(2):625-8. doi: 10.1016/0042-6822(90)90033-n.