PMID- 21611687 OWN - NLM STAT- MEDLINE DCOM- 20120127 LR - 20211020 IS - 1432-184X (Electronic) IS - 0095-3628 (Linking) VI - 62 IP - 3 DP - 2011 Oct TI - Effects of continuous thermophilic composting (CTC) on bacterial community in the active composting process. PG - 599-608 LID - 10.1007/s00248-011-9882-z [doi] AB - The method of continuous thermophilic composting (CTC) remarkably shortened the active composting cycle and enhanced the compost stability. Effects of CTC on the quantities of bacteria, with a comparison to the traditional composting (TC) method, were explored by plate count with incubation at 30, 40 and 50 degrees C, respectively, and by quantitative PCR targeting the universal bacterial 16S rRNA genes and the Bacillus 16S rRNA genes. The comparison of cultivatable or uncultivatable bacterial numbers indicated that CTC might have increased the biomass of bacteria, especially Bacillus spp., during the composting. Denaturing gradient gel electrophoresis (DGGE) analysis was employed to investigate the effects of CTC on bacterial diversity, and a community dominated by fewer species was detected in a typical CTC run. The analysis of sequence and phylogeny based on DGGE indicated that the continuously high temperature had changed the structure of bacterial community and strengthened the mainstay role of the thermophilic and spore-forming Bacillus spp. in CTC run. FAU - Xiao, Yong AU - Xiao Y AD - College of Environmental Science and Engineering, Hunan University, Changsha 410082, China. xiaoyong1982@msn.com FAU - Zeng, Guang-Ming AU - Zeng GM FAU - Yang, Zhao-Hui AU - Yang ZH FAU - Ma, Yan-He AU - Ma YH FAU - Huang, Cui AU - Huang C FAU - Shi, Wen-Jun AU - Shi WJ FAU - Xu, Zheng-Yong AU - Xu ZY FAU - Huang, Jing AU - Huang J FAU - Fan, Chang-Zheng AU - Fan CZ LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20110525 PL - United States TA - Microb Ecol JT - Microbial ecology JID - 7500663 RN - 0 (DNA, Bacterial) RN - 0 (RNA, Ribosomal, 16S) RN - 0 (Soil) SB - IM MH - Bacillus/genetics/*growth & development MH - Biomass MH - Colony Count, Microbial MH - DNA, Bacterial/genetics MH - Denaturing Gradient Gel Electrophoresis MH - *Hot Temperature MH - Phylogeny MH - RNA, Ribosomal, 16S/genetics MH - Refuse Disposal/*methods MH - *Soil MH - *Soil Microbiology EDAT- 2011/05/26 06:00 MHDA- 2012/01/28 06:00 CRDT- 2011/05/26 06:00 PHST- 2010/05/04 00:00 [received] PHST- 2011/05/14 00:00 [accepted] PHST- 2011/05/26 06:00 [entrez] PHST- 2011/05/26 06:00 [pubmed] PHST- 2012/01/28 06:00 [medline] AID - 10.1007/s00248-011-9882-z [doi] PST - ppublish SO - Microb Ecol. 2011 Oct;62(3):599-608. doi: 10.1007/s00248-011-9882-z. Epub 2011 May 25.