PMID- 2161358
OWN - NLM
STAT- MEDLINE
DCOM- 19900712
LR  - 20190828
IS  - 0020-711X (Print)
IS  - 0020-711X (Linking)
VI  - 22
IP  - 5
DP  - 1990
TI  - Guanine nucleotides have a direct inhibitory effect on polyphosphoinositide 
      turnover in rat cortical synaptosomes.
PG  - 507-11
AB  - 1. The possible involvement of guanosine 5'-triphosphate (GTP)-binding proteins 
      in the receptor mediated polyphosphoinositide (PPI) turnover event was 
      investigated in rat cortical synaptosomes. 2. It was studied under the effects of 
      guanine nucleotides on 32Pi incorporation into synaptosomal phospholipids in the 
      absence or presence of carbachol. 3. The basal 32Pi incorporation into these 
      phospholipids was altered by the presence of 1 mM carbachol: i.e. a decrease in 
      32Pi incorporation into phosphatidylinositol-4,5-bisphosphate and 
      phosphatidylinositol-4-phosphate and an increase in the incorporation of 32Pi 
      into phosphatidylinositol and phosphatidic acid. 4. In the presence of guanine 
      nucleotides: GTP, Gpp(NH)p and GDP at suitable concentrations, there was a 
      general decreasing effect on 32Pi incorporation into all 4 phospholipids, which 
      are all involved in PPI turnover cycle, either in the basal or 
      carbachol-stimulated levels. 5. There was no selective effect among the guanine 
      nucleotides studied on this PPI turnover event. It is, therefore, likely that 
      these nucleotides have a direct inhibitory effect on PPI turnover, and this 
      action may not act through a GTP-binding protein.
FAU - Wei, J W
AU  - Wei JW
AD  - Institute of Neurosciences, National Yang-Ming Medical College, Taipei, Taiwan, 
      Republic of China.
FAU - Hung, W C
AU  - Hung WC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Biochem
JT  - The International journal of biochemistry
JID - 0250365
RN  - 0 (Guanine Nucleotides)
RN  - 0 (Phosphates)
RN  - 0 (Phosphatidic Acids)
RN  - 0 (Phosphatidylinositol 4,5-Diphosphate)
RN  - 0 (Phosphatidylinositol Phosphates)
RN  - 0 (Phosphatidylinositols)
RN  - 0 (phosphatidylinositol 4-phosphate)
RN  - 146-91-8 (Guanosine Diphosphate)
RN  - 34273-04-6 (Guanylyl Imidodiphosphate)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 8Y164V895Y (Carbachol)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Carbachol/pharmacology
MH  - Cerebral Cortex/drug effects/*metabolism
MH  - GTP-Binding Proteins/metabolism
MH  - Guanine Nucleotides/*pharmacology
MH  - Guanosine Diphosphate/pharmacology
MH  - Guanosine Triphosphate/pharmacology
MH  - Guanylyl Imidodiphosphate/pharmacology
MH  - Phosphates/metabolism
MH  - Phosphatidic Acids/metabolism
MH  - Phosphatidylinositol 4,5-Diphosphate
MH  - Phosphatidylinositol Phosphates
MH  - Phosphatidylinositols/*metabolism
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Synaptosomes/drug effects/*metabolism
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1016/0020-711x(90)90265-5 [doi]
PST - ppublish
SO  - Int J Biochem. 1990;22(5):507-11. doi: 10.1016/0020-711x(90)90265-5.