PMID- 21615420
OWN - NLM
STAT- MEDLINE
DCOM- 20120309
LR  - 20111118
IS  - 1365-2605 (Electronic)
IS  - 0105-6263 (Linking)
VI  - 34
IP  - 6 Pt 2
DP  - 2011 Dec
TI  - Impaired expression and distribution of adherens and gap junction proteins in the 
      seminiferous tubules of rats undergoing autoimmune orchitis.
PG  - e566-77
LID - 10.1111/j.1365-2605.2011.01165.x [doi]
AB  - Experimental autoimmune orchitis (EAO) is characterized by an interstitial 
      lymphomononuclear cell infiltration and a severe lesion of seminiferous tubules 
      (ST) with germ cells that undergo apoptosis and sloughing. The aim of this study 
      was to analyse the expression and localization of adherens junction (AJ) 
      proteins: N-cadherin, alpha-, beta- and p120 catenins and gap junction protein, connexin 
      43 (Cx43), to explore some aspects of germ-cell sloughing during the development 
      of orchitis. EAO was induced in Sprague-Dawley adult rats by active immunization 
      with testicular homogenate and adjuvants. Control rats (C) were injected with 
      saline solution and adjuvants. Concomitant with early signs of germ-cell 
      sloughing, we observed by immunofluorescence and Western blot, a delocalization 
      and a significant increase in N-cadherin and alpha-catenin expression in the ST of 
      EAO compared with C rats. In spite of this increased AJ protein expression, a 
      severe germ-cell sloughing occurred. This is probably due to the impairment of 
      the AJ complex function, as shown by the loss of N-cadherin/beta-catenin 
      colocalization (confocal microscopy) and increased pY654 beta-catenin expression, 
      suggesting lower affinity of these two proteins and increased pERK1/2 expression 
      in the testis of EAO rats. The significant decrease in Cx43 expression detected 
      in EAO rats suggests a gap junction function impairment also contributing to 
      germ-cell sloughing.
CI  - (c) 2011 The Authors. International Journal of Andrology (c) 2011 European Academy of 
      Andrology.
FAU - Perez, C
AU  - Perez C
AD  - Instituto de Investigaciones en Reproduccion, Facultad de Medicina, Universidad 
      de Buenos Aires, Buenos Aires, Argentina. ceciliaperez77@yahoo.com.ar
FAU - Sobarzo, C
AU  - Sobarzo C
FAU - Jacobo, P
AU  - Jacobo P
FAU - Jarazo Dietrich, S
AU  - Jarazo Dietrich S
FAU - Theas, M
AU  - Theas M
FAU - Denduchis, B
AU  - Denduchis B
FAU - Lustig, L
AU  - Lustig L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110525
PL  - England
TA  - Int J Androl
JT  - International journal of andrology
JID - 8000141
RN  - 0 (Connexins)
SB  - IM
MH  - Adherens Junctions/*metabolism
MH  - Animals
MH  - Autoimmune Diseases/*metabolism
MH  - Connexins/*metabolism
MH  - Male
MH  - Orchitis/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seminiferous Tubules/*metabolism
EDAT- 2011/05/28 06:00
MHDA- 2012/03/10 06:00
CRDT- 2011/05/28 06:00
PHST- 2011/05/28 06:00 [entrez]
PHST- 2011/05/28 06:00 [pubmed]
PHST- 2012/03/10 06:00 [medline]
AID - 10.1111/j.1365-2605.2011.01165.x [doi]
PST - ppublish
SO  - Int J Androl. 2011 Dec;34(6 Pt 2):e566-77. doi: 10.1111/j.1365-2605.2011.01165.x. 
      Epub 2011 May 25.