PMID- 21618228
OWN - NLM
STAT- MEDLINE
DCOM- 20120105
LR  - 20110915
IS  - 1096-9861 (Electronic)
IS  - 0021-9967 (Linking)
VI  - 519
IP  - 16
DP  - 2011 Nov 1
TI  - Involvement of Galpha(olf)-expressing neurons in the vomeronasal system of Bufo 
      japonicus.
PG  - 3189-201
LID - 10.1002/cne.22671 [doi]
AB  - Most terrestrial vertebrates possess anatomically distinct olfactory organs: the 
      olfactory epithelium (OE) and the vomeronasal organ (VNO). In rodents, olfactory 
      receptors coupled to Galpha(olf) are expressed in the OE, whereas vomeronasal 
      receptors type 1 (V1R) and vomeronasal receptors type 2 (V2R), coupled to Galpha(i2) 
      and Galpha(o) , respectively, are expressed in the VNO. These receptors and G 
      proteins are thought to play important roles in olfactory perception. However, we 
      previously reported that only V2R and Galpha(o) expression is detected in the Xenopus 
      laevis VNO. As X. laevis spends its entire life in water, we considered that 
      expression of limited types of chemosensory machinery in the VNO might be due to 
      adaptation of the VNO to aquatic life. Thus, we analyzed the expression of G 
      proteins in the VNO and the accessory olfactory bulb (AOB) of the adult Japanese 
      toad, Bufo japonicus, because this species is well adapted to a terrestrial life. 
      By using immunohistochemical analysis in combination with in situ hybridization 
      and DiI labeling, we found that B. japonicus Galpha(olf) and Galpha(o) were expressed in 
      the apical and middle-to-basal layer of the vomeronasal neuroepithelium, and that 
      the axons of these Galpha(olf) - and Galpha(o) -expressing vomeronasal neurons projected 
      to the rostral and caudal accessory olfactory bulb, respectively. These results 
      strongly suggest that both the Galpha(olf) - and Galpha(o) -mediated signal transduction 
      pathways function in the B. japonicus VNO. The expression of Galpha(olf) in the B. 
      japonicus VNO may correlate with the detection of airborne chemical cues and with 
      a terrestrial life.
CI  - Copyright (c) 2011 Wiley-Liss, Inc.
FAU - Hagino-Yamagishi, Kimiko
AU  - Hagino-Yamagishi K
AD  - Integrated Neuroscience Research Project, The Tokyo Metropolitan Institute of 
      Medical Science, Tokyo 156-8506, Japan. yamagishi-km@igakuken.or.jp
FAU - Nakazawa, Hideo
AU  - Nakazawa H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (GTP-Binding Protein alpha Subunits)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Bufonidae
MH  - Female
MH  - GTP-Binding Protein alpha Subunits/analysis/*biosynthesis
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Male
MH  - Olfactory Receptor Neurons/*metabolism
MH  - Signal Transduction/physiology
MH  - Vomeronasal Organ/*metabolism
EDAT- 2011/05/28 06:00
MHDA- 2012/01/06 06:00
CRDT- 2011/05/28 06:00
PHST- 2011/05/28 06:00 [entrez]
PHST- 2011/05/28 06:00 [pubmed]
PHST- 2012/01/06 06:00 [medline]
AID - 10.1002/cne.22671 [doi]
PST - ppublish
SO  - J Comp Neurol. 2011 Nov 1;519(16):3189-201. doi: 10.1002/cne.22671.