PMID- 21618258
OWN - NLM
STAT- MEDLINE
DCOM- 20121101
LR  - 20161125
IS  - 1099-1263 (Electronic)
IS  - 0260-437X (Linking)
VI  - 32
IP  - 8
DP  - 2012 Aug
TI  - Experience with local lymph node assay performance standards using standard 
      radioactivity and nonradioactive cell count measurements.
PG  - 590-6
LID - 10.1002/jat.1684 [doi]
AB  - The local lymph node assay (LLNA) is the preferred test for identification of 
      skin-sensitizing substances by measuring radioactive thymidine incorporation into 
      the lymph node. To facilitate acceptance of nonradioactive variants, validation 
      authorities have published harmonized minimum performance standards (PS) that the 
      alternative endpoint assay must meet. In the present work, these standards were 
      applied to a variant of the LLNA based on lymph node cell counts (LNCC) run in 
      parallel as a control with the standard LLNA with radioactivity measurements, 
      with threshold concentrations (EC3) being determined for the sensitizers. Of the 
      22 PS chemicals tested in this study, 21 yielded the same results from standard 
      radioactivity and cell count measurements; only 2-mercaptobenzothiazole was 
      positive by LLNA but negative by LNCC. Of the 16 PS positives, 15 were positive 
      by LLNA and 14 by LNCC; methylmethacrylate was not identified as sensitizer by 
      either of the measurements. Two of the six PS negatives tested negative in our 
      study by both LLNA and LNCC. Of the four PS negatives which were positive in our 
      study, chlorobenzene and methyl salicylate were tested at higher concentrations 
      than the published PS, whereas the corresponding concentrations resulted in 
      consistent negative results. Methylmethacrylate and nickel chloride tested 
      positive within the concentration range used for the published PS. The results 
      indicate cell counts and radioactive measurements are in good accordance within 
      the same LLNA using the 22 PS test substances. Comparisons with the published PS 
      results may, however, require balanced analysis rather than a simple checklist 
      approach.
CI  - Copyright (c) 2011 John Wiley & Sons, Ltd.
FAU - Basketter, David
AU  - Basketter D
AD  - DABMEB Consultancy Ltd, Sharnbrook, UK.
FAU - Kolle, Susanne N
AU  - Kolle SN
FAU - Schrage, Arnhild
AU  - Schrage A
FAU - Honarvar, Naveed
AU  - Honarvar N
FAU - Gamer, Armin O
AU  - Gamer AO
FAU - van Ravenzwaay, Bennard
AU  - van Ravenzwaay B
FAU - Landsiedel, Robert
AU  - Landsiedel R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20110525
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (Chlorobenzenes)
RN  - 0 (Salicylates)
RN  - 196OC77688 (Methylmethacrylate)
RN  - 696BNE976J (nickel chloride)
RN  - 7OV03QG267 (Nickel)
RN  - K18102WN1G (chlorobenzene)
RN  - LAV5U5022Y (methyl salicylate)
SB  - IM
MH  - Animals
MH  - Biological Assay/*methods/*standards
MH  - Cell Count/standards
MH  - Chlorobenzenes/adverse effects/analysis
MH  - Dermatitis, Allergic Contact/metabolism/*pathology
MH  - Endpoint Determination
MH  - Female
MH  - Linear Models
MH  - *Local Lymph Node Assay
MH  - Methylmethacrylate/adverse effects/analysis
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Nickel/adverse effects/analysis
MH  - Radioactivity
MH  - Salicylates/adverse effects/analysis
EDAT- 2011/05/28 06:00
MHDA- 2012/11/02 06:00
CRDT- 2011/05/28 06:00
PHST- 2011/02/16 00:00 [received]
PHST- 2011/03/04 00:00 [revised]
PHST- 2011/03/04 00:00 [accepted]
PHST- 2011/05/28 06:00 [entrez]
PHST- 2011/05/28 06:00 [pubmed]
PHST- 2012/11/02 06:00 [medline]
AID - 10.1002/jat.1684 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2012 Aug;32(8):590-6. doi: 10.1002/jat.1684. Epub 2011 May 25.